Mouse Uromodulin ELISA Kit, Chemiluminescent
- SimpleStep Ignite™ ELISA
- Recombinant
- 각 태그의 뜻
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- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.05 - 120 ng/ml with a sensitivity of 0.02ng/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending
- sELISA
Lab
Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
- sELISA
Lab
Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
Dynamic range comparison between the Chemiluminescent Mouse Uromodulin SimpleStep ELISA, colorimetric Mouse Uromodulin SimpleStep ELISA (ab245726), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.
- sELISA
Lab
Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
Interpolated concentration of native Uromodulin was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Lab
Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
Standard curve comparison between the Chemiluminescent Mouse Uromodulin SimpleStep ELISA and colorimetric Mouse Uromodulin SimpleStep ELISA (ab245726) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
Interpolated concentrations of native Uromodulin in mouse kidney tissue extract based on a 100 µg/mL extract load. The concentrations of Uromodulin were measured in duplicate and interpolated from the Uromodulin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Uromodulin concentration was determined to be 41624 pg/mL in kidney tissue extract.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
Interpolated concentrations of native Uromodulin in mouse urine. The concentrations of Uromodulin were measured in duplicates interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 1 : 400. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Uromodulin concentration was determined to be 12867 ng/mL in urine.
- sELISA
Supplier Data
Sandwich ELISA - Mouse Uromodulin ELISA Kit, Chemiluminescent (AB325735)
Interpolated concentrations of spiked Uromodulin in cell culture media. The concentrations of Uromodulin were measured in duplicates interpolated from the Uromodulin standard curves and corrected for sample dilution. Undiluted samples are as follows : cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2).
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제품 세부 정보
Mouse Uromodulin ELISA Kit, Chemiluminescent (ab325735 ) is a rapid single-wash sandwich 55-min ELISA to measure Mouse Uromodulin in Serum, EDTA Plasma, Urine, Cell culture media, Tissue Extracts, Citrate plasma with a sensitivity of 0.02 ng/mL.
How the assay works
Mouse Uromodulin ELISA Kit, Chemiluminescent (ab325735 ) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.
Assay Specificity
Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Mouse Uromodulin (ab325735 ) protocol summary
1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Uromodulin antibody [EPR22133-150] - BSA and Aside free (Capture) ab259581
- Anti-Uromodulin antibody [EPR22133-142] - BSA and Aside free (Detector) ab259582
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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