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AB238262

Mouse YKL-40 / CHI3L1 ELISA Kit

Mouse YKL-40 / CHI3L1 ELISA 키트

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(2 제품이 사용된 논문 )

Mouse YKL-40 / CHI3L1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Mouse YKL-40 / CHI3L1 with a sensitivity of 3.17 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
  • ELISA

Supplier Data

ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)

Mouse CHI3L1 Competitor Std Curve Comparison.

Standard Curve comparison between Mouse CHI3L1 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)

Example of mouse CHI3L1 standard curve in Sample Diluent NS. The CHI3L1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)

Interpolated concentrations of native CHI3L1 in mouse supernatant samples. The concentrations of CHI3L1 were measured in duplicates, interpolated from the CHI3L1 standard curves and corrected for sample dilution. Undiluted samples are as follows : mouse heart supernatant 50%, mouse liver supernatant 50%, mouse lung supernatant 2.5%, and mouse heart supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHI3L1 concentration was determined to be 6.31 ng/mL in mouse heart supernatant, 1.65 ng/mL in mouse liver supernatant), 157.17 pg/mL in mouse lung supernatant, and 23.91 ng/mL in mouse spleen supernatant.

Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)
  • sELISA

Supplier Data

Sandwich ELISA - Mouse YKL-40 / CHI3L1 ELISA Kit (AB238262)

Interpolated concentrations of native CHI3L1 in mouse serum and plasma samples. The concentrations of CHI3L1 were measured in duplicates, interpolated from the CHI3L1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 5%, plasma (EDTA) 5% and plasma (heparin) 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CHI3L1 concentration was determined to be 58.00 ng/mL in serum, 28.30 ng/mL in plasma (citrate), 52.70 pg/mL in plasma (EDTA), and 82.22 ng/mL in plasma (Heparin).

주요 정보

검출 방식

Colorimetric

샘플 타입

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Mouse

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 3.17 pg/mL

검출 범위

54.69 - 3500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Mouse YKL-40 / CHI3L1 ELISA Kit ab238262 is a rapid single-wash 90-min Sandwich ELISA to measure Mouse YKL-40 / CHI3L1 in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 3.17 pg/mL.

How the assay works

Mouse YKL-40 / CHI3L1 SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Mouse YKL-40 / CHI3L1 ELISA Kit ab238262 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-YKL-40/CHI3L1 antibody [EPR21754-8] - BSA and Azide free (Capture) ab245069
- Anti-YKL-40/CHI3L1 antibody [EPR21754-27] - BSA and Azide free (Detector) ab245069
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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "106 - 112 %", "average": "= 109" }, { "sample": "Serum", "range": "106 - 112 %", "average": "= 108" }, { "sample": "EDTA Plasma", "range": "106 - 107 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "96 - 103 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "96 - 99 %", "average": "= 98" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab238262-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB238262-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB238262-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB238262-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB238262-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB238262-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB238262-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB238262-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse YKL-40 / CHI3L1 Capture Antibody", "number":"AB238262-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Mouse YKL-40 / CHI3L1 Lyophilized Recombinant Protein", "number":"AB238262-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Mouse YKL-40 / CHI3L1 Detector Antibody", "number":"AB238262-CMP07", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

YKL-40 also known as chitinase-3-like protein 1 (CHI3L1) is a glycoprotein with a molecular weight of approximately 40 kDa. Scientists often study it for its roles in inflammatory processes. It has expressions in numerous tissues including liver cartilage and the central nervous system. The CHI3L1 protein does not possess chitinase activity unlike other members of the chitinase family yet it interacts with various ligands to influence cellular behavior.
Biological function summary

YKL-40 plays significant roles in tissue remodeling and inflammation. It does not form part of a larger complex by itself but influences several cellular processes. This protein affects cell proliferation migration and survival making it involved in both normal physiological processes and pathological conditions. CHI3L1 also exhibits a connection to the immune response where it modulates macrophage activity and interacts with other immune cells.

Pathways

YKL-40 is integral to the inflammatory and extracellular matrix pathways. It associates with proteins such as interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-?) influencing signaling cascades that modulate inflammation. Through these interactions CHI3L1 becomes a mediator of connective tissue metabolism and immune reactions in various pathophysiological states.

YKL-40 shows strong links to conditions like osteoarthritis and certain cancers. In osteoarthritis elevated levels of CHI3L1 reflect increased cartilage degradation while in oncology it correlates with tumor angiogenesis and metastasis potential. Its connection with proteins such as matrix metalloproteinases highlights its role in disease mechanisms highlighting its potential as a biomarker for early detection and disease progression monitoring.
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제품 프로토콜

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타겟 정보

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.
See full target information Chi3l1

대체 명칭 보기

Brp39, Chil1, Chi3l1, Chitinase-3-like protein 1, BRP39 protein, Breast regression protein 39, Cartilage glycoprotein 39, CGP-39, GP-39
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Brain, behavior, & immunity - health 26:100555 PubMed36457825

2022

Conserved YKL-40 changes in mice and humans after postoperative delirium.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer David-Bercholz,Leah Acker,Ana I Caceres,Pau Yen Wu,Saanvi Goenka,Nathan O Franklin,Ramona M Rodriguiz,William C Wetsel,Michael Devinney,Mary Cooter Wright,Henrik Zetterberg,Ting Yang,Miles Berger,Niccolò Terrando

Biomedicines 10: PubMed35625766

2022

Role of Chitinase-3-like Protein 1 in Cardioprotection and Angiogenesis by Post-Infarction Exercise Training.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Li,Fangnan Wu,Lei Xi,Zhenjun Tian
제품이 사용된 논문 모두 보기
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websiteProtocolBooklet
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Product promise

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