N Cadherin ELISA Kit

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The N Cadherin Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213756) is designed for the quantitative measurement of Human N-Cadherin in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Cadherin-2/N-Cadherin has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: D160-A724) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cadherin-2/N Cadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Cadherin-2/N-Cadherin amount of sample captured in plate.

N Cadherin, also known as Cadherin-2 (CDH2), is a protein that in humans is encoded by the CDH2 gene. It is a classical cadherin from the cadherin superfamily. This gene is mapped to 18q12.1. N-Cadherin is expressed in the brain, skeletal and cardiac muscle. N Cadherin is commonly found in cancer cells and provides a mechanism for transendothelial migration. It is a calcium dependent cell-cell adhesion glycoprotein comprising five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.