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AB273256

Ovine IL-17A ELISA Kit

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Ovine IL-17A ELISA Kit is a Sandwich ELISA for the measurement of Ovine IL-17A in Sheep in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Ovine IL-17A ELISA Kit (AB273256)
  • sELISA

Supplier Data

Sandwich ELISA - Ovine IL-17A ELISA Kit (AB273256)

Ovine IL-17A ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Sheep

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.45 pg/mL

검출 범위

0.41 - 100 pg/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

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Ovine IP-10 ELISA Kit

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9869

Anti-GADD34 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GADD34 antibody (AB9869)

  • 3
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Ovine IL-17A ELISA Kit (ab273256) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Ovine IL-17A in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Ovine IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Ovine IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "104 - 121 %", "average": "= 110.1" }, { "sample": "Plasma", "range": "82 - 139 %", "average": "= 105.1" }, { "sample": "Cell culture media", "range": "92 - 105 %", "average": "= 98.12" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273256-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273256-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273256-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273256-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273256-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Ovine IL-17A coated Microplate (12 x 8 wells)", "number":"AB273256-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB273256-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Ovine IL-17A Antibody", "number":"AB273256-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Ovine IL-17A Standard (Lyophilized)", "number":"AB273256-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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제품 프로토콜

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타겟 정보

See full target information IL17A

대체 명칭 보기

CTLA-8, Cytotoxic T lymphocyte associated protein 8, Cytotoxic T lymphocyte associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17_HUMAN, Interleukin 17 (cytotoxic T lymphocyte associated serine esterase 8), Interleukin-17A, Interleukin17, OTTHUMP00000016597, OTTMUSP00000046003
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websiteProtocolBooklet
en
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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