Phospho-Glucocorticoid Receptor (Ser226) and Total Glucocorticoid Receptor ELISA Kit
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Sandwich ELISA - Phospho-Glucocorticoid Receptor (Ser226) and Total Glucocorticoid Receptor ELISA Kit (AB279811)
A549 cells treated with Calyculin A and PMA. A549 cells were treated with Calyculin A and PMA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-Glucocorticoid Receptor (Ser226) and Total Glucocorticoid Receptor ELISA Kit (AB279811)
Positive Control.
A431 cells were treated with recombinant human EGF at 37°C for 20 min.
Cells were solubilzed at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-Glucocorticoid Receptor (Ser226) and Total Glucocorticoid Receptor ELISA Kit (AB279811)
A549 cells treated with Calyculin A and PMA. A549 cells were treated with Calyculin A and PMA.
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Phospho-Glucocorticoid Receptor (S226) and Total Glucocorticoid Receptor ELISA Kit (ab279811) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Glucocorticoid Receptor protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Glucocorticoid Receptor and total Glucocorticoid Receptor. An anti-pan Glucocorticoid Receptor antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Glucocorticoid Receptor present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho GR (S226) antibody is added to detect phosphorylated Glucocorticoid Receptor. In the remaining wells, biotinylated anti-pan GR antibody is used to detect pan Glucocorticoid Receptor. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Glucocorticoid Receptor (S226) or pan Glucocorticoid Receptor bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The glucocorticoid receptor plays a significant role in mediating the physiological effects of glucocorticoids. It controls processes like inflammation and immune response. The receptor is part of a larger receptor complex enhancing its effectiveness in gene regulation. It modulates expression levels of diverse genes involved in metabolism immune functionality and cellular growth. GR's regulatory actions make it contextual within various biological processes impacting cellular behavior extensively.
Pathways
The glucocorticoid receptor integrates into significant signaling networks like the hypothalamic-pituitary-adrenal (HPA) axis and the inflammatory response pathway. This receptor coordinates with other proteins to control stress responses and inflammatory signals. In the HPA axis GR helps regulate cortisol levels and counteracts inflammatory cytokines. Its interaction with other receptors and transcription factors exemplifies its role in essential pathways that maintain homeostasis and stress adaptation within the organism.
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