AB279822

Phospho-Hsp27 (S82) ELISA Kit

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Phospho-Hsp27 (S82) ELISA Kit is a ELISA for the measurement of Phospho-Hsp27 (S82) in Human in Cell/Tissue Extracts samples.

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Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

sELISA

Supplier Data

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

Positive Control.

HeLa cells were treated with PMA at 37°C for 20 mins.

Solubilize cells at 4 x 10⁷ cells/ml in Cell Lysate Buffer.

Serial dilutions of lysates were analyzed in this ELISA.

sELISA

Supplier Data

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

HeLa cells treated/untreated with PMA. HeLa cells were untreated or treated with 250 nM PMA for 20 mins.

sELISA

Supplier Data

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

HeLa cells treated/untreated with PMA. HeLa cells were untreated or treated with 250 nM PMA for 20 mins.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate

Reacts with

Human

결과 유형

Semi-Quantitative

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Phospho-Hsp27 (S82) ELISA Kit (ab279822) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Hsp27 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-Hsp27. An anti-pan Hsp27 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Hsp27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-Hsp27 (S82) antibody is used to detect phosphorylated Hsp27. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Hsp27 (S82) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279822-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Positive Control - treated HeLa cell lysate", "number":"AB279822-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279822-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pan-Phospho-Hsp27 Coated Microplate", "number":"AB279822-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279822-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279822-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279822-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279822-CMP07", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279822-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-Hsp27 (S82)-antibody", "number":"AB279822-CMP05", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Hsp27 also known as HSPB1 is a small heat shock protein with a molecular weight of approximately 27 kilodaltons. This protein is expressed in various tissues including muscle heart and brain. It functions as a molecular chaperone that stabilizes unfolded proteins preventing their aggregation. Hsp27 undergoes phosphorylation at specific residues which modulates its chaperone activity and interaction with other proteins.

Biological function summary

Hsp27 plays a critical role in cellular stress response by regulating actin cytoskeleton dynamics and inhibiting apoptosis. It forms part of a complex that includes other proteins such as alphaB-crystallin. This complex facilitates the reorganization of proteins under stress conditions enhancing cell survival during oxidative stress or thermal shock. Hsp27 also modulates inflammatory responses and has been shown to affect cell migration.

Pathways

Hsp27 integrates into the apoptosis and inflammation pathways. It interacts with apoptotic machinery such as caspase proteins to protect cells by hindering apoptosome formation. Additionally Hsp27 can engage with pathways involving the nuclear factor-kappa B (NF-kB) impacting inflammatory signaling. CPTC (carboxyl-pyrene-trioctylamine) can modulate these pathways by altering Hsp27 function and interactions.

Hsp27 has connections to neurodegenerative diseases and cancer. In neurodegenerative conditions such as Alzheimer's disease its chaperone activity is thought to protect neurons from misfolded protein aggregates. In cancer Hsp27 supports tumor cell survival and resistance to chemotherapy by interacting with proteins like Akt and p53. These interactions highlight the complex role of Hsp27 in modulating cellular responses in various pathological states.

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제품 프로토콜

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타겟 정보

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state (PubMed : 10383393, PubMed : 20178975). Plays a role in stress resistance and actin organization (PubMed : 19166925). Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins (PubMed : 23728742).

See full target information HSPB1 pS82

대체 명칭 보기

HSP27, HSP28, HSPB1, Heat shock protein beta-1, HspB1, 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, Heat shock 27 kDa protein, Heat shock protein family B member 1, Stress-responsive protein 27, HSP 27, SRP27

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websiteProtocolBooklet

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Product promise

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Phospho-Hsp27 (S82) ELISA Kit

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

Sandwich ELISA - Phospho-Hsp27 (S82) ELISA Kit (AB279822)

주요 정보

검출 방식

샘플 타입

Reacts with

결과 유형

분석 플랫폼

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise