Phospho-MSK2 / RSK-B (S360) and Total MSK2 / RSK-B ELISA Kit
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Supplier Data
Sandwich ELISA - Phospho-MSK2 / RSK-B (S360) and Total MSK2 / RSK-B ELISA Kit (AB279867)
HeLa cells treated with/without Calyculin A.
HeLa cells were untreated or treated with 0.1 μg/ml Calyculin A for 30 minutes.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-MSK2 / RSK-B (S360) and Total MSK2 / RSK-B ELISA Kit (AB279867)
Positive Control.
HeLa cells were treated with Calyculin A at 37°C for 30 min.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-MSK2 / RSK-B (S360) and Total MSK2 / RSK-B ELISA Kit (AB279867)
HeLa cells treated with/without Calyculin A.
HeLa cells were untreated or treated with 0.1 μg/ml Calyculin A for 30 minutes.
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Phospho-MSK2 / RSK-B (S360) and Total MSK2 / RSK-B ELISA Kit (ab279867) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated MSK2 / RSK-B protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-MSK2 / RSK-B and total MSK2 / RSK-B. An anti-pan MSK2 / RSK-B antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and MSK2 / RSK-B present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho MSK2 / RSK-B (S360) antibody is added to detect phosphorylated MSK2 / RSK-B. In the remaining wells, biotinylated anti-pan-MSK2 / RSK-B antibody is used to detect pan MSK2 / RSK-B. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP- conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MSK2 / RSK-B (S360) or pan MSK2 / RSK-B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This kinase involves itself in the regulation of transcription by phosphorylating CREB and activating histone acetylation. MSK2 acts in concert with other kinases and proteins forming part of larger signaling complexes that modulate gene expression and chromatin states. It also impacts inflammation by activating specific pathways that target inflammatory gene expression serving as a connection point between various cellular signals and responses.
Pathways
MSK2 plays a significant role in the MAPK signaling pathway and the NF-kB pathway. These pathways are pivotal in cell differentiation proliferation and stress responses. MSK2 often functions in conjunction with proteins like ERK1/2 and p38 MAPK facilitating the downstream effects of these signaling molecules. In particular MSK2's phosphorylation activities have direct effects on transcription factors within these pathways impacting cellular fate decisions.
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