Phospho-p95/NBS1 (S343) and Total p95/NBS1 ELISA Kit
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Sandwich ELISA - Phospho-p95/NBS1 (S343) and Total p95/NBS1 ELISA Kit (AB279872)
T47D cells treated/untreated with UV. T47D cells were untreated or treated with UV.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-p95/NBS1 (S343) and Total p95/NBS1 ELISA Kit (AB279872)
Positive Control.
T47D cells were irradiated with UV.
Cells were solubilzed at 4 x 107 cells/ml in Cell Lysate Buffer.
Serial dilutions of lysates were analyzed in this ELISA.
- sELISA
Supplier Data
Sandwich ELISA - Phospho-p95/NBS1 (S343) and Total p95/NBS1 ELISA Kit (AB279872)
T47D cells treated/untreated with UV. T47D cells were untreated or treated with UV.
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Phospho-p95/NBS1 (S343) and Total p95/NBS1 ELISA Kit (ab279872) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated p95/NBS1 (S343) protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-p95/NBS1 and total p95/NBS1. An anti-pan p95/NBS1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and p95/NBS1 present in a sample is bound to the wells by the immobilized antibody and the wells are washed. In select wells, rabbit anti-phospho p95/NBS1 (S343) antibody is added to detect phosphorylated p95/NBS1. In the remaining wells, mouse anti-pan-p95/NBS1 antibody is used to detect pan p95/NBS1. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-mouse IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of p95/NBS1 (S343) or pan p95/NBS1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The NBS1 protein functions as an essential component of the MRN complex which also includes MRE11 and RAD50 proteins. This complex is fundamental in accurately detecting DNA double-strand breaks and initiating repair processes. Through its actions NBS1 facilitates numerous cellular processes that preserve genomic integrity such as homologous recombination and non-homologous end joining. Its influence in controlling the cell cycle further emphasizes its role in maintaining cellular health.
Pathways
The NBS1 protein engages significantly in the DNA damage response and repair pathways notably impacting the ATM signaling pathway. By interacting with proteins such as ATM kinase NBS1 facilitates the phosphorylation and activation of several downstream effectors necessary for DNA repair and cell cycle checkpoints. The MRN complex also links with the RAD50 protein highlighting its involvement in these pathways.
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