AB115343

Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit

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Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit is a Sandwich ELISA for the measurement of Phospho S232 PDH E1 alpha protein (PDHA1) Profiling in in Cell/Tissue Extracts samples from a wide range of species.

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ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

ELISA

Supplier Data

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

HeLa cells were cultured for 4 hours in media supplemented with DCA (20mM) to specifically inhibit mitochondrial PDH kinases, or NaF (20mM), a general inhibitor of serine/threonine protein phosphatases. The DCA treatment reduced the level of phospho S232. Conversely NaF treatment, to inhibit cellular serine phosphatases, increased the phosphorylation level of S232.

ELISA

Supplier Data

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

Example control sample curve for HepG2 cell extract. The phosphorylation state of PDHA1 can vary by treatment but also by cell culture conditions such as media supplements, nutrients and also cell density.

ELISA

Supplier Data

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

Example control sample curve for HeLa cell extract. The phosphorylation state of PDHA1 can vary by treatment but also by cell culture conditions such as media supplements, nutrients and also cell density.

ELISA

Supplier Data

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

The PDHA1 bound from undosed HeLa cells was subject to in-well kinase treatment (PDK1&3) or in-well phosphatase treatment (PDP1) according to the supplementary protocol shown below. Untreated cells showed a significant endogenous phosphorylation signal at S232 which is only slightly increased by kinase treatment. Conversely phosphatase treatment was able to significantly reduce the phospho S232 signal from the endogenous levels.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture extracts, Tissue Extracts

Reacts with

Mouse, Rat, Cow, Human

분석 유형

Sandwich

결과 유형

Qualitative

Sensitivity

= 31 µg/mL

검출 범위

31 - 1000 µg/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

ab115343 is an in vitro enzyme-linked immunosorbent assay to determine the levels of phospho S232 PDHA1 protein in cell and tissue lysates. The assay employs a mouse antibody specific for PDHA1 protein coated on a 96-well plate. Samples are pipetted into the wells and PDHA1 protein present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a rabbit anti-phospho S232 PDHA1 protein detector antibody is added. After washing away unbound detector antibody, HRP-conjugated anti-rabbit antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of phospho S232 PDHA1 protein bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

Store all components at 4°C. The kits are stable for at least 6

months. Unused microplate strips should be returned to the pouch

containing the desiccant and resealed.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7.7" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.3" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab115343-1x96Tests", "assetComponentDetails": [ { "size":"1 x 0.7 mL", "name":"10X phospho S232 PDHA1 protein Detector Antibody", "number":"AB115343-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"10X Blocking Buffer", "number":"AB115343-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"Extraction Buffer (ab260490)", "number":"AB115343-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP Development Solution", "number":"AB115343-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate 96 antibody coated wells in 12 strips", "number":"AB115343-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"20X Buffer", "number":"AB115343-CMP04", "productcode":"" }, { "size":"1 x 1 mL", "name":"10X HRP Label", "number":"AB115343-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PDHA1 also known as the pyruvate dehydrogenase E1 alpha subunit plays a mechanical role in cellular metabolism. It forms part of the larger pyruvate dehydrogenase (PDH) complex where it serves as a critical catalytic component. PDHA1 is expressed ubiquitously across different tissue types reflecting its fundamental function in energy production. The molecular weight of the PDHA1 protein is approximately 43 kDa. Alternate names for this protein include the PDH E1 component and it partners closely with other components in the PDH complex to facilitate its role.

Biological function summary

PDHA1 engages in the conversion of pyruvate into acetyl-CoA an important step in cellular respiration. This protein is part of the PDH complex which consists of multiple copies of three catalytic and two regulatory subunits. The conversion process is essential for linking glycolysis to the citric acid cycle efficiently channeling energy substrates within the cell. Furthermore the functional activity of PDHA1 is regulated through phosphorylation by the pyruvate dehydrogenase kinases (PDKs) and dephosphorylation by PDH phosphatases.

Pathways

PDHA1 is integral to the metabolic pathway of cellular respiration and energy production. It enables the transition between glycolysis and the citric acid cycle by facilitating the conversion of pyruvate to acetyl-CoA which enters the citric acid cycle. Related proteins in this pathway include PDHA2 and the regulatory PDKs that modulate PDHA1 activity. These interactions ensure energy metabolism adapts to various cellular conditions influencing energy balance and substrate utilization.

Mutations or dysfunctions in PDHA1 can lead to disorders such as pyruvate dehydrogenase deficiency and Leigh syndrome. These conditions result from impaired energy metabolism leading to severe neurological symptoms and overall energy deficits in tissues with high metabolic demands. The link between PDHA1 and diseases highlights the importance of maintaining its function. Additionally altered interaction with proteins involved in phosphorylation such as the PDKs can exacerbate pathogenic conditions by further disbalancing metabolic activities.

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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 12:837 PubMed34489398

2021

Mitochondrial dysfunction induces radioresistance in colorectal cancer by activating [Ca]-PDP1-PDH-histone acetylation retrograde signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Shi,You Wang,Huangang Jiang,Xuehua Sun,Hui Xu,Xue Wei,Yan Wei,Guohui Xiao,Zhiyin Song,Fuxiang Zhou

Oncology letters 21:176 PubMed33574915

2021

Phosphorylated form of pyruvate dehydrogenase α1 mediates tumor necrosis factor α-induced glioma cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Zijun Yang,Yidan Wang,Li Zhang,Chenjin Zhao,Donglin Wang

Molecular systems biology 13:953 PubMed29109155

2017

Temporal fluxomics reveals oscillations in TCA cycle flux throughout the mammalian cell cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Eunyong Ahn,Praveen Kumar,Dzmitry Mukha,Amit Tzur,Tomer Shlomi

PloS one 12:e0179672 PubMed28617852

2017

Dicumarol inhibits PDK1 and targets multiple malignant behaviors of ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjia Zhang,Jing Su,Huadan Xu,Shanshan Yu,Yanan Liu,Yong Zhang,Liankun Sun,Ying Yue,Xiaoli Zhou

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

ELISA - Phospho S232 PDH E1 alpha protein (PDHA1) Profiling ELISA Kit (AB115343)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

Precision

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise