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AB279947

Phospho-STAT6 (Y641) ELISA Kit

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Phospho-STAT6 (Y641) ELISA Kit is a ELISA for the measurement of Phospho-STAT6 (Y641) in Human in Cell/Tissue Extracts samples.
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Sandwich ELISA - Phospho-STAT6 (Y641) ELISA Kit (AB279947)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-STAT6 (Y641) ELISA Kit (AB279947)

Positive Control.

A431 cells were treated with recombinant human EGF at 37°C for 20 min.

Cells were solubilzed at 4 x 107 cells/ml in lysis buffer.

Serial dilutions of lysates were analyzed in this ELISA.

Sandwich ELISA - Phospho-STAT6 (Y641) ELISA Kit (AB279947)
  • sELISA

Supplier Data

Sandwich ELISA - Phospho-STAT6 (Y641) ELISA Kit (AB279947)

A431 cells treated with/without EGF.

A431 cells were treated or untreated with EGF.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate

Reacts with

Human

결과 유형

Semi-Quantitative

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Assay Kits

AB279948

Phospho-STAT6 (Y641) and Total STAT6 ELISA Kit

Sandwich ELISA - Phospho-STAT6 (Y641) and Total STAT6 ELISA Kit (AB279948)

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Primary Antibodies

AB263947

Anti-STAT6 (phospho Y641) antibody [EPR22599-78]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (AB263947)

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Proteins & Peptides

AB125625

Recombinant Human STAT6 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human STAT6 protein (AB125625)

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  • 0
Primary Antibodies

AB32520

Anti-STAT6 antibody [YE361]

20ul selling size|Recombinant|Advanced Validation|RabMAb|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT6 antibody [YE361] (AB32520)

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Phospho-STAT6 (Y641) ELISA Kit (ab279947) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated STAT6 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-STAT6. An anti-pan STAT6 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-phospho-STAT6 (Y641) antibody is used to detect phosphorylated STAT6. After washing away unbound antibody, HRP conjugated anti-rabbit IgG is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STAT6 (Y641) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279947-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Pan-STAT6 Coated Microplate", "number":"AB279947-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB279947-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Positive Control - A431 cell lysate", "number":"AB279947-CMP01", "productcode":"" }, { "size":"1 x 5 mL", "name":"2X Cell lysate buffer", "number":"AB279947-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB279947-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB279947-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB279947-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit anti-phospho-STAT6 (Y641)-antibody", "number":"AB279947-CMP07", "productcode":"" }, { "size":"1 x 25 µL", "name":"HRP-conjugated anti-rabbit IgG concentrate (500X)", "number":"AB279947-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

STAT6 also known as Signal Transducer and Activator of Transcription 6 is a transcription factor with a mass of around 94 kDa. This protein is an important component in the signaling pathways that respond to cytokines especially those of the IL-4 and IL-13 receptors. STAT6 mainly expresses in immune cells including T cells B cells and macrophages. It exists in the cytoplasm until the cytokine stimulation prompts its phosphorylation and translocation to the nucleus where it influences gene expression.
Biological function summary

STAT6 plays a central role in the development and function of Th2 cells a subset of T helper cells. The protein controls genes linked to the immune response and inflammation such as IL-4 and IL-13 by binding to specific DNA sequences as part of the transcriptional complex. These processes are critical for antibody class switching in B cells and also for regulating the differentiation of T cells which are processes important in defending against parasitic infections and are linked to allergies.

Pathways

STAT6 functions mainly in the JAK-STAT signaling pathway a pathway important for transmitting information from chemical signals outside cells to the cell nucleus resulting in DNA transcription. Within this pathway STAT6 works closely with JAK1 and JAK3 kinases which phosphorylate STAT6 enabling its activation. The protein also connects with other members of the STAT family like STAT1 and STAT3 during overlapping signaling processes.

STAT6 has associations with asthma and certain types of cancer such as colorectal cancer. In asthma STAT6 influences the production of IgE and eosinophilic inflammation often connected to allergic responses. Meanwhile STAT6 promotes tumorigenesis in some cancers by regulating genes responsible for cell survival and proliferation. It connects with proteins like IL-4 and IL-13 in these disorders which suggests potential therapeutic targets for treatment.
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제품 프로토콜

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타겟 정보

Carries out a dual function : signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
See full target information STAT6 pY641

대체 명칭 보기

Signal transducer and activator of transcription 6, IL-4 Stat, STAT6
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websiteProtocolBooklet
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Product promise

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