- sELISA
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Sandwich ELISA - Phosphotyrosine JAK1 ELISA Kit (AB279836)
Jurkat cells treated with/without Pervanadate.
Jurkat cells were treated with Pervanadate at 37°C for 10 mins.
- sELISA
Supplier Data
Sandwich ELISA - Phosphotyrosine JAK1 ELISA Kit (AB279836)
Positive Control.
Jurkat cells were treated with Pervanadate at 37°C for 10 mins.
Solubilize cells at 4 x 107 cells/ml in lysis buffer.
Serial dilutions of lysates were analyzed in this ELISA.
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Phosphotyrosine JAK1 ELISA Kit (ab279836) is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated JAK1 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-JAK1. An anti-pan JAK1 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated JAK1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody HRP-conjugated Streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-JAK1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This enzyme participates significantly in the immune response and hematopoiesis by being part of the JAK-STAT signaling pathway complex. When activated JAK1 phosphorylates specific tyrosine residues on the receptor creating docking sites for STAT proteins which then get phosphorylated by JAK1. This phosphorylation allows STAT proteins to dimerize and translocate to the nucleus affecting gene expression involved in growth survival and differentiation processes.
Pathways
JAK1 is importantly engaged in the JAK-STAT pathway and the Interferon signaling pathway. Within these pathways JAK1 collaborates closely with other proteins like JAK2 TYK2 and STAT proteins. It acts as a mediator of the cellular response to a variety of cytokines and growth factors playing a role in the regulation of immune responses and cell proliferation.
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