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AB190536

Pig IgA ELISA Kit

5

(2 리뷰들)

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(4 제품이 사용된 논문 )

Pig IgA ELISA Kit is a Sandwich ELISA for the measurement of Pig IgA in Pig in Biofluids samples.
1 이미지
Sandwich ELISA - Pig IgA ELISA Kit (AB190536)
  • sELISA

Supplier Data

Sandwich ELISA - Pig IgA ELISA Kit (AB190536)

Representative standard curve using ab190536 IgG Pig ELISA Kit.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Serum

Reacts with

Pig

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 4.38 ng/mL

검출 범위

12.5 - 400 ng/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's IgA Pig ELISA kit (ab190536) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgA in pig serum and plasma.

In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": null, "average": "> 85" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab190536-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB190536-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB190536-CMP06", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB190536-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB190536-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IgA Pig Calibrator (Lyophilized)", "number":"AB190536-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IgA Pig Antibody coated microwells", "number":"AB190536-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"IgA Pig HRP Conjugate", "number":"AB190536-CMP05", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Please refer to protocols
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.
Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.
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제품 프로토콜

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타겟 정보

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Animals : an open access journal from MDPI 11: PubMed34438677

2021

Effects of Coated Cysteamine on Oxidative Stress and Inflammation in Weaned Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Wang,Miaomiao Bai,Kang Xu,Yirui Shao,Zhe Yang,Xia Xiong,Ruilin Huang,Yao Li,Hongnan Liu

Food science & nutrition 9:1112-1120 PubMed33598195

2021

Alternative antibiotic feed additives alleviate pneumonia with inhibiting ACE-2 expression in the respiratory system of piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Wen Lu,Sheue-Er Wang,Wan-Jhen Wu,Li-Yu Su,Che-Hsuan Wang,Pei-Hwa Wang,Chung-Hsin Wu

Frontiers in immunology 11:1924 PubMed33013844

2020

Yeast as a Protein Source for Weaned Piglets-Impact on Immune Response and Gut Microbiota.

Applications

Unspecified application

Species

Unspecified reactive species

Leidy Lagos,Alexander Kashulin Bekkelund,Adrijana Skugor,Ragnhild Ånestad,Caroline P Åkesson,Charles McL Press,Margareth Øverland

Journal of the science of food and agriculture 99:3616-3627 PubMed30628086

2019

Effects of pulverized oyster mushroom (Pleurotus ostreatus) on diarrhea incidence, growth performance, immunity, and microbial composition in piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Seidu Adams,Dongsheng Che,Jiang Hailong,Bao Zhao,Han Rui,Kofi Danquah,Guixin Qin
제품이 사용된 논문 모두 보기
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