AB100754

Pig IL-1 beta ELISA Kit

Name: Pig IL-1 beta ELISA Kit
Brand: Abcam Limited
SKU: AB100754
Rating: 4 (3 reviews)

(3 리뷰들)

(7 제품이 사용된 논문 )

Pig IL-1 beta ELISA Kit is a Sandwich ELISA for the measurement of Pig IL-1 beta in Pig in Biofluids, Cell Culture Media samples.

1 이미지

Sandwich ELISA - Pig IL-1 beta ELISA Kit (AB100754)

sELISA

Supplier Data

Sandwich ELISA - Pig IL-1 beta ELISA Kit (AB100754)

Representative Standard Curve using ab100754

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Pig

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 6 pg/mL

검출 범위

8.23 - 6000 pg/mL

분석 플랫폼

Microplate

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

제품 세부 정보

Abcam's IL1 beta Pig ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of pig IL1 beta in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for IL1 beta coated on a 96-well plate. Standards and samples are pipetted into the wells and IL1 beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-pig IL1 beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL1 beta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "89 - 104 %", "average": "= 96.91" }, { "sample": "Serum", "range": "88 - 105 %", "average": "= 97.32" }, { "sample": "Plasma", "range": "80 - 92 %", "average": "= 87.75" } ]

style

left-column

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100754-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100754-CMP05", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB100754-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100754-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"IL1 beta Microplate (12 x 8 wells)", "number":"AB100754-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100754-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100754-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB100754-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Pig IL-1 beta", "number":"AB100754-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Pig IL-1 beta Standard (lyophilized)", "number":"AB100754-CMP02", "productcode":"" } ] } } }

style

left-column

특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

style

left-column

style

left-column

제품 프로토콜

style

left-column

타겟 정보

Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis : its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore.

See full target information IL1B

대체 명칭 보기

Interleukin-1 beta, IL-1 beta, IL1B

style

left-column

제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 21:667 PubMed37752585

2023

Tumor microenvironment in a minipig model of spinal cord glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Muhibullah S Tora,Stewart G Neill,Yuliya Lakhina,Hemza Assed,Michelle Zhang,Purva P Nagarajan,Thais Federici,Juanmarco Gutierrez,Kimberly B Hoang,Yuhong Du,Kecheng Lei,Nicholas M Boulis

Cell communication and signaling : CCS 18:23 PubMed32046726

2020

Immune response in piglets orally immunized with recombinant Bacillus subtilis expressing the capsid protein of porcine circovirus type 2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Zhang,Chunxiao Mou,Yanan Cao,En Zhang,Qian Yang

Marine drugs 17: PubMed31835381

2019

Epinecidin-1 Protects against Methicillin Resistant Infection and Sepsis in Pyemia Pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Han-Ning Huang,Chieh-Yu Pan,Bor-Chyuan Su,Hung-Yi Wu,Jyh-Yih Chen

Journal of applied microbiology 128:355-365 PubMed31618501

2019

Effects of dietary calcium pyruvate on gastrointestinal tract development, intestinal health and growth performance of newly weaned piglets fed low-protein diets.

Applications

Unspecified application

Species

Unspecified reactive species

K Wan,Y Li,W Sun,R An,Z Tang,L Wu,H Chen,Z Sun

Biology of reproduction 102:475-488 PubMed31616930

2019

Ablation of conceptus PTGS2 expression does not alter early conceptus development and establishment of pregnancy in the pig†.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline A Pfeiffer,Ashley E Meyer,Kelsey E Brooks,Paula R Chen,Jessica Milano-Foster,Lee D Spate,Joshua A Benne,Raissa F Cecil,Melissa S Samuel,Lauren A Ciernia,Christine M Spinka,Michael F Smith,Kevin D Wells,Thomas E Spencer,Randall S Prather,Rodney D Geisert

Biology of reproduction 101:148-161 PubMed31066888

2019

New perspective on conceptus estrogens in maternal recognition and pregnancy establishment in the pig†.

Applications

Unspecified application

Species

Unspecified reactive species

Ashley E Meyer,Caroline A Pfeiffer,Kelsey E Brooks,Lee D Spate,Joshua A Benne,Raissa Cecil,Melissa S Samuel,Clifton N Murphy,Susanta Behura,Megan K McLean,Lauren A Ciernia,Michael F Smith,Kristin M Whitworth,Kevin D Wells,Thomas E Spencer,Randall S Prather,Rodney D Geisert

Journal of animal science 96:4723-4730 PubMed30476156

2018

Efficacy of energy supplementation on growth performance and immune response of suckling pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Naiana Einhardt Manzke,Bruna Kuhn Gomes,Eduardo Gonçalves Xavier,Gustavo Julio Mello Monteiro de Lima

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Pig IL-1 beta ELISA Kit

Sandwich ELISA - Pig IL-1 beta ELISA Kit (AB100754)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise