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AB272467

Pig IL-13 ELISA Kit

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Pig IL-13 ELISA Kit is a single-wash 90-min Simplestep used to quantify Pig IL-13 with a sensitivity of 5.45 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - Pig IL-13 ELISA Kit (AB272467)
  • sELISA

Supplier Data

Sandwich ELISA - Pig IL-13 ELISA Kit (AB272467)

Example of pig IL-13 standard curve in Sample Diluent NS.

The IL-13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Pig IL-13 ELISA Kit (AB272467)
  • sELISA

Supplier Data

Sandwich ELISA - Pig IL-13 ELISA Kit (AB272467)

Interpolated concentrations of spike IL-13 in pig serum, plasma, and cell culture media.

The concentrations of IL-13 were measured in duplicates, interpolated from the target standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (heparin) 25%, plasma (EDTA) 50%, and cell culture media (RPMI 1640 containing 10% fetal bovine serum) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Pig

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 5.45 pg/mL

검출 범위

18.8 - 1200 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Pig IL-13 ELISA kit (ab272467) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pig IL-13 protein in serum, plasma and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Pig IL-13 with 5.5 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Interleukin-13 (IL-13) is a secreted immunoregulatory cytokine in the IL-4/IL-13 family that is linked to allergic reactions and cancer pathogenesis. IL-13 inhibits inflammatory cytokine production and promotes immunoglobulin class switching on B-cells. IL-13 is linked to allergic rhinitis, a common disease of complex inheritance characterized by mucosal inflammation caused by allergen exposure.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 90 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "77 - 84 %", "average": "= 80" }, { "sample": "Cell culture media", "range": "94 - 98 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "86 - 92 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "72 - 77 %", "average": "= 75" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272467-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB272467-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272467-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272467-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272467-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272467-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272467-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB272467-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Pig IL-13 Capture Antibody", "number":"AB272467-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Pig IL-13 Lyophilized Recombinant Protein", "number":"AB272467-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Pig IL-13 Detector Antibody", "number":"AB272467-CMP08", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Cytokine that plays important roles in allergic inflammation and immune response to parasite infection. Synergizes with IL2 in regulating interferon-gamma synthesis. Stimulates B-cell proliferation, and activation of eosinophils, basophils, and mast cells (By similarity). Plays an important role in controlling IL33 activity by modulating the production of transmembrane and soluble forms of interleukin-1 receptor-like 1/IL1RL1 (By similarity). Displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF through a mechanism that partially involves suppression of NF-kappa-B (By similarity). Also functions on nonhematopoietic cells, including endothelial cells where it induces vascular cell adhesion protein 1/VCAM1, which is important in the recruitment of eosinophils. Exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, to activate JAK1 and TYK2, leading to the activation of STAT6. Aside from IL13RA1, another receptor IL13RA2 acts as a high affinity decoy for IL13 and mediates internalization and depletion of extracellular IL13 (By similarity).
See full target information IL13

대체 명칭 보기

Interleukin-13, IL-13, IL13
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websiteProtocolBooklet
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Product promise

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