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AB318928

Pig Lipocalin-2/NGAL ELISA Kit

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(0 출판물)

Pig Lipocalin-2/NGAL ELISA Kit is a single-wash 90-min Simplestep used to quantify Pig Lipocalin-2/NGAL with a sensitivity of 4.144 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
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Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Interpolated concentrations of pig Lipocalin-2/NGAL in Göttingen minipig serum in 96-well vs. 384-well plates. Interpolated concentration of native Lipocalin-2/NGAL was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 400 Göttingen mini pig serum.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Example of pig Lipocalin-2/NGAL standard curve in Sample Diluent NS. Example of pig Lipocalin-2/NGAL standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Interpolated concentrations of pig Lipocalin-2/NGAL in Göttingen minipig serum and Yorkshire pig serum. Interpolated concentration of native Lipocalin-2/NGAL was measured in duplicate at different sample concentrations. Undiluted samples are as follows : minipig serum 1 : 400 and Yorkshire pig serum 1 : 1,000.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Interpolated concentrations of pig Lipocalin-2/NGAL in minipig plasma (citrate), minipig plasma (EDTA), and minipig plasma (heparin). Interpolated concentration of native Lipocalin-2/NGAL was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 100 minipig plasma (citrate), minipig plasma (EDTA), and minipig plasma (heparin).  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)
  • sELISA

Supplier Data

Sandwich ELISA - Pig Lipocalin-2/NGAL ELISA Kit (AB318928)

Example of pig Lipocalin-2/NGAL standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of pig Lipocalin-2/NGAL standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, EDTA Plasma, Cell culture media, Citrate plasma, Heparin Plasma

Reacts with

Pig

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 4.144 pg/mL

검출 범위

12.5 - 800 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Pig Lipocalin-2/NGAL SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lipocalin-2/NGAL protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Pig Lipocalin-2/NGAL with 4.144 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.6" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.2" } ]
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Recovery

[ { "sample": "Serum", "range": "108 - 118 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "93 - 104 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "107 - 114 %", "average": "= 111" }, { "sample": "Heparin Plasma", "range": "94 - 107 %", "average": "= 101" }, { "sample": "Cell culture media", "range": "94 - 96 %", "average": "= 95" } ]
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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab318928-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB318928-CMP10" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB318928-CMP08" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318928-CMP06" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB318928-CMP07" }, { "size":"20 x 1 Vial", "name":"Pig Lipocalin-2/NGAL Lyophilized Recombinant Protein", "number":"AB318928-CMP03" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318928-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318928-CMP05" }, { "size":"10 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Capture Antibody", "number":"AB318928-CMP01" }, { "size":"10 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Detector Antibody", "number":"AB318928-CMP02" }, { "size":"10 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318928-CMP09" } ] }, "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab318928-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB318928-CMP10" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB318928-CMP08" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318928-CMP06" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB318928-CMP07" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318928-CMP04" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318928-CMP05" }, { "size":"2 x 1 Vial", "name":"Pig Lipocalin-2/NGAL Lyophilized Recombinant Protein", "number":"AB318928-CMP03" }, { "size":"1 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Capture Antibody", "number":"AB318928-CMP01" }, { "size":"1 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Detector Antibody", "number":"AB318928-CMP02" }, { "size":"1 x 384 Wells", "name":"384 well CaptSure™ microplates", "number":"AB318928-CMP11" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab318928-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB318928-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB318928-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB318928-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB318928-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Pig Lipocalin-2/NGAL Lyophilized Recombinant Protein", "number":"AB318928-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB318928-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB318928-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Capture Antibody", "number":"AB318928-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Pig Lipocalin-2/NGAL Detector Antibody", "number":"AB318928-CMP02", "productcode":"" }, { "size":"1 x 96 Wells", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB318928-CMP09", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

See full target information Lipocalin-2

대체 명칭 보기

LCN2, NGAL
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websiteProtocolBooklet
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Product promise

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