AB316263

Prostaglandin E2 ELISA Kit - Extracellular

Prostaglandin E2 ELISA 키트 - Extracellular

SimpleStep
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Prostaglandin E2 ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify Prostaglandin E2 with a sensitivity of 4.75 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

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Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Serum from nine individual healthy female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Prostaglandin E2 concentration was determined to be 234 pg/mL with a range of 92 – 585 pg/mL.

cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Interpolated concentration of native Prostaglandin E2 was measured in duplicate at different sample concentrations. Undiluted samples are 50% Human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent ES.

cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Prostaglandin E2 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent ES. Undiluted samples are 50% Human urine, and cell culture media with or without 10% FBS. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Example of Prostaglandin E2 standard curve. OD (450 nm) values (mean +/- SD) are graphed.

cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Prostaglandin E2 was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent ES. Undiluted samples are 50% Human serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

cELISA

Supplier Data

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Serum from ten individual healthy male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Prostaglandin E2 concentration was determined to be 335 pg/mL with a range of 67 – 837 pg/mL.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, EDTA Plasma, Urine, Citrate plasma, Heparin Plasma, Cell culture media

분석 유형

Competitive

결과 유형

Quantitative

Sensitivity

= 4.75 pg/mL

검출 범위

23.438 - 1500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Prostaglandin E2 SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of Prostaglandin E2 in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Urine, and Cell culture media. Quantitate Prostaglandin E2 with 4.75 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.5" }, { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 13.9" } ]

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Recovery

[ { "sample": "Serum", "range": "83 - 117 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "93 - 110 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "88 - 101 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "83 - 90 %", "average": "= 86" }, { "sample": "Urine", "range": "88 - 104 %", "average": "= 96" }, { "sample": "Cell culture media", "range": "107 - 109 %", "average": "= 108" } ]

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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab316263-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB316263-CMP01" }, { "size":"10 x 80 µL", "name":"50X Prostaglandin E2 HRP Conjugate", "number":"AB316263-CMP10" }, { "size":"10 x 1 Vial", "name":"Lyophilized Prostaglandin E2 CaptSure Conjugate", "number":"AB316263-CMP11" }, { "size":"10 x 2 Vial", "name":"Prostaglandin E2 Lyophilized Standard", "number":"AB316263-CMP09" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB316263-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316263-CMP06" }, { "size":"1 x 200 mL", "name":"Sample Diluent ES", "number":"AB316263-CMP07" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB316263-CMP03" }, { "size":"10 x 8 mL", "name":"Antibody Diluent CPI2", "number":"AB316263-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316263-CMP02" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316263-96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB316263-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Prostaglandin E2 HRP Conjugate", "number":"AB316263-CMP10", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Lyophilized Prostaglandin E2 CaptSure Conjugate", "number":"AB316263-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Prostaglandin E2 Lyophilized Standard", "number":"AB316263-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent ES", "number":"AB316263-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316263-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB316263-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB316263-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent CPI2", "number":"AB316263-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB316263-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316263-CMP06", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Prostaglandin E2 (PGE2) also known as dinoprostone is a lipid compound with a molecular weight of approximately 352.47 g/mol. This compound is synthesized in various tissues like the kidneys lungs and brain primarily by the enzymatic action of cyclooxygenase (COX) on arachidonic acid. As a member of the prostaglandin family PGE2 functions as an important signaling molecule and mediator of inflammation. Researchers often use PGE2 ELISA kits and assays including those specific for mouse PGE2 to measure its levels accurately in biological samples.

Biological function summary

As a potent mediator PGE2 plays roles in inflammation fever induction and regulation of vascular tone. It exerts its effects through specific G-protein-coupled receptors (EP1 EP2 EP3 and EP4) on the surface of target cells. Although not part of a strict protein complex PGE2 works in conjunction with other inflammatory mediators including cytokines and other prostaglandins. It influences cellular activities by modulating the intracellular cyclic AMP levels and calcium fluxes.

Pathways

PGE2 is an important component of the prostaglandin biosynthesis pathway and the broader inflammatory signal transduction pathway. Within these pathways it relates to the COX enzymes specifically COX-2 which converts arachidonic acid into PGH2 the precursor of PGE2. This metabolic pathway highlights the interplay between PGE2 and other prostanoids impacting inflammatory responses and homeostatic processes.

PGE2 has associations with conditions such as arthritis and cancer. Elevated levels of PGE2 are often found in inflamed tissues and have been linked to the pathogenesis of rheumatoid arthritis by promoting inflammation and pain. In cancer PGE2 can encourage tumoral growth by modulating the immune response and promoting angiogenesis. In these contexts the interactions of PGE2 with enzymes like COX-2 and cytokines such as tumor necrosis factor (TNF) further connect its roles to disease progression and potential therapeutic targets.

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제품 프로토콜

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타겟 정보

See full target information Prostaglandin E2

대체 명칭 보기

PGE2, Dinoprostone, Prostin E2

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in pharmacology 17:1706420 PubMed42051267

2026

LITAF suppresses breast cancer and paclitaxel resistance by ubiquitinating and degrading PCMT1 to inhibit COX-2-dependent arachidonic acid metabolism.

Applications

ELISA

Species

Human

Na Yang,Jingying Cao,Feng Jiang,Renxian Cao,Yiqi Liu

Scientific reports : PubMed42031984

2026

Gnetum montanum extract attenuates lipopolysaccharide induced acute lung inflammation through Nrf2 and heme oxygenase 1 mediated redox modulation in macrophages.

Applications

ELISA

Species

Mouse

Duc-Vinh Pham,Hong-Linh Tran,Thu-Hang Nguyen,Hong-Nhung Bui,Hai-Nam Nguyen,Thanh Nguyen Le,Thi Hien Pham,Thuy-Duong Nguyen

Journal of cosmetic dermatology 24:e70521 PubMed41118451

2025

New Insights Into Advanced Glycation End Products Induced Melanogenesis and Intervention Strategies.

Applications

ELISA

Species

Human

Xi Yang,Mengqi You,Huanjun Zhou,Zhen Li,Guangwen He,Thomas Mammone,Nadine Pernodet,Jian Cao

Cell death & disease 16:463 PubMed40541951

2025

ARNTL-mediated INO80-DHX15 axis reprograms the glycolytic metabolism and augments the progression of endometrial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Ye,Genyi Jiang,Yihan Sun,Bilan Li

Basic and clinical andrology 35:9 PubMed40055615

2025

Pelvic vasectomy and its protective effects on rat testis function.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Yang,Yujun Chen,Xiaofeng Cheng,Jingxin Wu,Ruohui Huang,Biao Qian,Gongxian Wang

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Prostaglandin E2 ELISA Kit - Extracellular

Prostaglandin E2 ELISA 키트 - Extracellular

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

Competitive ELISA - Prostaglandin E2 ELISA Kit - Extracellular (AB316263)

주요 정보

검출 방식

샘플 타입

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Secondary Antibodies

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise