AB325035
Protein L Ligand Leakage Detection ELISA Kit (ab325035 ) is a Sandwich ELISA to measure Protein L Ligand Leakage Detection in bioprocessing samples.
- Colorimetric ELISA kit - 450nm readout. Works on any standard plate reader
- For use on bioprocessing samples during the production and purification of antibody fragments
- Colorimetric ELISA kit - 450nm readout. Works on any standard plate reader
- For use on bioprocessing samples during the production and purification of antibody fragments
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- sELISA
Supplier Data
Sandwich ELISA - Protein L Ligand Leakage Detection ELISA Kit (AB325035)
Example standard curve for ab325035.
보완 제품
Reactivity 정보
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
제품 세부 정보
Protein L Ligand Leakage Detection ELISA Kit (ab325035) is a sandwich ELISA for the quantification of ligand leakage in eluates from Protein L affinity chromatography medium containing immunoglobulin (Ig) or Ig-fragments.
How the assay works
Microwells are pre-coated with anti-protein L Ligand antibodies to capture protein L ligand from samples and standards pipetted into the wells. Following binding, an anti protein L ligand-HRP conjugate is added and incubated. After washing to remove non-specific interactions, a ready-to-use TMB substrate is introduced, triggering a colorimetric reaction proportional to the concentration of protein L ligand in the sample. The reaction is halted by adding a stop solution, and absorbance is measured at 450 nm.
Protein L Ligand Leakage Detection ELISA Kit (ab325035 ) protocol summary
1. Add 50µL of standard or diluted samples to appropriate wells. Incubate for 60 min at room temperature
2. Wash
3. Add 100µL of Anti-Protein L Ligand :HRP Conjugate to each well. Incubate 60 min at room temperature
4. Wash
5. Add 100µL TMB substrate to each well. Incubate for 30 min in the dark
6. Add 100µL of Stop solution to each well
7. Read at 450 nm with 10-15 min.
How the assay works
Microwells are pre-coated with anti-protein L Ligand antibodies to capture protein L ligand from samples and standards pipetted into the wells. Following binding, an anti protein L ligand-HRP conjugate is added and incubated. After washing to remove non-specific interactions, a ready-to-use TMB substrate is introduced, triggering a colorimetric reaction proportional to the concentration of protein L ligand in the sample. The reaction is halted by adding a stop solution, and absorbance is measured at 450 nm.
Protein L Ligand Leakage Detection ELISA Kit (ab325035 ) protocol summary
1. Add 50µL of standard or diluted samples to appropriate wells. Incubate for 60 min at room temperature
2. Wash
3. Add 100µL of Anti-Protein L Ligand :HRP Conjugate to each well. Incubate 60 min at room temperature
4. Wash
5. Add 100µL TMB substrate to each well. Incubate for 30 min in the dark
6. Add 100µL of Stop solution to each well
7. Read at 450 nm with 10-15 min.
제품 구성
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특성 및 보관 정보
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Please refer to protocols
Product promise
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