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AB323606

RAB35 ELISA Kit

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RAB35 ELISA Kit is a single-wash 90-min Simplestep used to quantify RAB35 with a sensitivity of 0.086 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Sandwich ELISA - RAB35 ELISA Kit (AB323606)
  • sELISA

Supplier Data

Sandwich ELISA - RAB35 ELISA Kit (AB323606)

Example of human/mouse/rat RAB35 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - RAB35 ELISA Kit (AB323606)
  • sELISA

Supplier Data

Sandwich ELISA - RAB35 ELISA Kit (AB323606)

Example of RAB35 standard curve in 1X Cell Extraction Buffer PTR in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - RAB35 ELISA Kit (AB323606)
  • sELISA

Supplier Data

Sandwich ELISA - RAB35 ELISA Kit (AB323606)

Interpolated concentration of native RAB35 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human hippocampus tissue extract 50 µg/mL, mouse brain tissue extract 250 µg/mL, and rat brain tissue extract 40 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - RAB35 ELISA Kit (AB323606)
  • sELISA

Supplier Data

Sandwich ELISA - RAB35 ELISA Kit (AB323606)

Interpolated concentration of native RAB35 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Jurkat cell extract 150 µg/mL and MCF-7 cell extract 100 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - RAB35 ELISA Kit (AB323606)
  • sELISA

Supplier Data

Sandwich ELISA - RAB35 ELISA Kit (AB323606)

Interpolated concentration of native RAB35 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50 µg/mL human hippocampus tissue extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell Lysate, Tissue Extracts

Reacts with

Human, Mouse, Rat

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.086 ng/mL

검출 범위

0.242 - 15.5 ng/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Primary Antibodies

AB152138

Anti-RAB35 antibody

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RAB35 antibody (AB152138)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

RAB35 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RAB35 protein in Cell Lysate, Tissue extracts. Quantitate RAB35 with 0.086 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "98 - 105 %", "average": "= 103" }, { "sample": "Tissue Extracts", "range": "92 - 100 %", "average": "= 96" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (PubMed : 30905672). RAB35 is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane (PubMed : 21951725). During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge (PubMed : 16950109). May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes (By similarity).
See full target information RAB35

대체 명칭 보기

RAB1C, RAY, RAB35, Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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