- sELISA
Supplier Data
Sandwich ELISA - Rabbit IL-21 ELISA Kit (AB273246)
This standard curve is for demonstration only. A standard curve must be run with each assay.
Reactivity 정보
제품 세부 정보
Rabbit IL-21 ELISA Kit (ab273246) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit IL-21 in serum (rabbit IL-21 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as an anti-coagulant for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for rabbit IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- rabbit IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Precision
Recovery
제품 구성
특성 및 보관 정보
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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