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AB273246

Rabbit IL-21 ELISA Kit

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Rabbit IL-21 ELISA Kit is a Sandwich ELISA for the measurement of Rabbit IL-21 in Rabbit in Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Rabbit IL-21 ELISA Kit (AB273246)
  • sELISA

Supplier Data

Sandwich ELISA - Rabbit IL-21 ELISA Kit (AB273246)

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, Citrate plasma, EDTA Plasma, Serum

Reacts with

Rabbit

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 2.45 ng/mL

검출 범위

2.458 - 600 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

Rabbit IL-21 ELISA Kit (ab273246) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit IL-21 in serum (rabbit IL-21 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as an anti-coagulant for use in this assay) and cell culture supernatants.

This assay employs an antibody specific for rabbit IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- rabbit IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

Recovery

[ { "sample": "Serum", "range": "67 - 77 %", "average": "= 71.2" }, { "sample": "Cell culture media", "range": "140 - 148 %", "average": "= 144.6" }, { "sample": "Plasma", "range": "67 - 82 %", "average": "= 71.55" } ]

제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273246-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273246-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273246-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273246-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273246-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rabbit IL-21 coated Microplate (12 x 8 wells)", "number":"AB273246-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273246-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Rabbit IL-21 Antibody", "number":"AB273246-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit IL-21 Standard (Lyophilized)", "number":"AB273246-CMP02", "productcode":"" } ] } } }

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C

제품 프로토콜

websiteProtocolBooklet
en

Product promise

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