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AB273241

Rabbit Leptin ELISA Kit

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Rabbit Leptin ELISA Kit is a Sandwich ELISA for the measurement of Rabbit Leptin in Rabbit in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Rabbit Leptin ELISA Kit (AB273241)
  • sELISA

Supplier Data

Sandwich ELISA - Rabbit Leptin ELISA Kit (AB273241)

Rabbit Leptin ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Rabbit

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.2 ng/mL

검출 범위

0.205 - 50 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Secondary Antibodies

AB150110

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed

Immunohistochemistry (Frozen sections) - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 555) preadsorbed (AB150110)

  • 5
  • 2
Secondary Antibodies

AB150129

Donkey Anti-Goat IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (AB150129)

  • 5
  • 6
Secondary Antibodies

AB150064

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed (AB150064)

  • 5
  • 1
Secondary Antibodies

AB150111

Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed (AB150111)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Rabbit Leptin ELISA Kit (ab273241) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit Leptin in serum (Rabbit Leptin concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Rabbit Leptin coated on a 96-well plate. Standards and samples are pipetted into the wells and Leptin present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Rabbit Leptin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Leptin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 121 %", "average": "= 101.2" }, { "sample": "Plasma", "range": "67 - 109 %", "average": "= 88.68" }, { "sample": "Cell culture media", "range": "73 - 106 %", "average": "= 91.98" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273241-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273241-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273241-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB273241-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273241-CMP07", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273241-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rabbit Leptin coated Microplate (12 x 8 wells)", "number":"AB273241-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273241-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit Leptin Standard (Lyophilized)", "number":"AB273241-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Rabbit Leptin Antibody", "number":"AB273241-CMP05", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Leptin also known as the OB protein is a hormone with a molecular weight of approximately 16 kDa. Primarily it is produced by adipocytes in white adipose tissue and to a lesser extent in the placenta stomach and other tissues. This protein plays a significant role in energy homeostasis by conveying information to the central nervous system regarding fat storage. In scientific studies leptin is often measured using methods like the leptin ELISA kit or mouse leptin assay to monitor its levels and function.
Biological function summary

Leptin regulates food intake and energy expenditure by binding to its receptors in the hypothalamus. It is an important part of the feedback loop to manage body weight and appetite. Leptin is not part of a larger protein complex and acts directly on its target tissues. By signal transduction pathways it influences cellular processes such as metabolism and even impacts fertility and immune responses.

Pathways

Leptin is involved in the neuroendocrine signaling pathway and the MAPK/ERK pathway. In the neuroendocrine system leptin interacts with proteins like proopiomelanocortin (POMC) and neuropeptide Y (NPY) to manage appetite and energy use. Additionally it activates the JAK/STAT pathway when binding to its receptor which influences gene expression involved in metabolism and inflammation.

Leptin is linked to obesity and type 2 diabetes. Leptin deficiencies or resistance can lead to metabolic disorders as the hormone's signaling is disrupted affecting energy balance and storage. Its connection to adiponectin another hormone derived from fat tissue is critical in understanding these conditions; while leptin promotes inflammation adiponectin has anti-inflammatory effects. Exploring these proteins' roles and their balance offers insights into treatments for obesity-related disorders.
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제품 프로토콜

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타겟 정보

See full target information Leptin
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websiteProtocolBooklet
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Product promise

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