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AB273250

Rabbit NCAM1 ELISA Kit (CD56)

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Rabbit NCAM1 ELISA Kit (CD56) is a Sandwich ELISA for the measurement of Rabbit NCAM1 (CD56) in Rabbit in Biofluids, Cell Culture Media samples.
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Sandwich ELISA - Rabbit NCAM1 ELISA Kit (CD56) (AB273250)
  • sELISA

Supplier Data

Sandwich ELISA - Rabbit NCAM1 ELISA Kit (CD56) (AB273250)

This standard curve is for demonstration only. A standard curve must be run with each assay.

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Rabbit

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 0.45 ng/mL

검출 범위

0.41 - 100 ng/mL

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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보완 제품

Reagents, Controls & Accessories

AB206978

Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA®

SimpleStep

ELISA - Pre-Coated 96 Well Microplate (12 x 8 well strips) SimpleStep ELISA® (AB206978)

  • 0
  • 0
Primary Antibodies

AB64693

Anti-CD206 antibody

BOND RX™ Validated

Western blot - Anti-CD206 antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB237709

Anti-CD8 alpha antibody [CAL66]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL66] (AB237709)

  • 5
  • 2
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Rabbit NCAM1 ELISA Kit (CD56) (ab273250) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit NCAM1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for rabbit NCAM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NCAM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-rabbit NCAM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NCAM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "136 - 145 %", "average": "= 140.8" }, { "sample": "Plasma", "range": "134 - 147 %", "average": "= 140.4" }, { "sample": "Cell culture media", "range": "87 - 120 %", "average": "= 105.9" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273250-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273250-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB273250-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273250-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273250-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Rabbit NCAM1 (CD56) coated Microplate (12 x 8 wells)", "number":"AB273250-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rabbit NCAM1 (CD56) Standard (Lyophilized)", "number":"AB273250-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Rabbit NCAM1 (CD56) Antibody", "number":"AB273250-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin Concentrate", "number":"AB273250-CMP08", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NCAM1 also known as Neural Cell Adhesion Molecule 1 or CD56 is an important cell surface glycoprotein that plays a role in cell-cell adhesion. It has a molecular mass of approximately 120-140 kDa. NCAM1 is widely expressed in various tissues including the nervous system skeletal muscle and certain hematopoietic cells. In flow cytometry and immunohistochemistry NCAM1's expression is extensively studied to track and analyze cell populations using techniques like NCAM1 ELISA for quantification.
Biological function summary

NCAM1 influences neuronal growth survival and synaptic plasticity. This protein is part of the immunoglobulin superfamily and contributes to dynamic interactions during development. NCAM1 exists on cell membranes assisting in the formation and maintenance of tissue structures. In particular cell adhesion molecule CAL53 interacts with NCAM1 highlighting its involvement in synaptic connections. Additionally NCAM1 impacts cellular migration and differentiation particularly in myogenic processes involving MY31's pathway.

Pathways

The NCAM1 protein participates in the MAPK signaling pathway and the PI3K/AKT pathway both essential in cellular growth and survival. NCAM1 acts alongside ERIC1 aiding in the transduction of signals that regulate cytoskeletal rearrangement. These pathways influence cellular communication and responses to external stimuli further enhancing tissue development and regeneration mechanisms.

NCAM1 is associated with neurodegenerative conditions like Alzheimer's disease and certain types of cancer including neuroblastoma. Changes in NCAM1 expression or function can disrupt normal cell adhesion and signaling leading to pathological states. The protein's interaction with cell surface markers such as CD56 mouse marker helps in the diagnosis and therapy of these disorders providing potential therapeutic targets or biomarkers for clinical applications.
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제품 프로토콜

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타겟 정보

See full target information NCAM2
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websiteProtocolBooklet
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Product promise

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