Rat alpha 2 Macroglobulin ELISA Kit
- Recombinant
- SimpleStep
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- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- sELISA
Supplier Data
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)
Interpolated concentrations of rat alpha 2 Macroglobulin in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 20,000, plasma (citrate) 1 : 10,000, plasma (EDTA) 1 : 10,000, and plasma (heparin) 1 : 10,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)
Interpolated concentrations of rat alpha 2 Macroglobulin in serum in 96-well vs. 384-well plates. Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted sample is 1 : 20,000 serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)
Interpolated concentrations of rat alpha 2 Macroglobulin in urine. Interpolated concentration of native alpha 2 Macroglobulin was measured in duplicate at different sample concentrations. Undiluted sample is 50% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)
Interpolated concentrations of alpha 2 Macroglobulin in rat, human, mouse, cow, and monkey serum samples. Serial dilutions of rat, human, mouse, cow, and monkey serum samples were measured in duplicate and interpolated from the rat standard curve. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
- sELISA
Supplier Data
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)
Example of rat alpha 2 Macroglobulin standard curve in Sample Diluent NS. Example of rat alpha 2 Macroglobulin standard curve. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Rat alpha 2 Macroglobulin ELISA Kit (AB320793)
Example of rat alpha 2 Macroglobulin standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Example of rat alpha 2 Macroglobulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity 정보
제품 세부 정보
Rat alpha 2 Macroglobulin SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of alpha 2 Macroglobulin protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media, Urine. Quantitate Rat alpha 2 Macroglobulin with 14 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.
Precision
Recovery
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보관 정보
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This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.
Pathways
Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.
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