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AB100775

Rat CXCL5 ELISA Kit

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Rat CXCL5 ELISA Kit is a Sandwich ELISA for the measurement of Rat CXCL5 in Rat in Cell/Tissue Extracts samples.
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Sandwich ELISA - Rat CXCL5 ELISA Kit (AB100775)
  • sELISA

Unknown

Sandwich ELISA - Rat CXCL5 ELISA Kit (AB100775)

Representative standard curve using ab100775

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture extracts, Tissue Extracts

Reacts with

Rat

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 15 pg/mL

검출 범위

8.23 - 6000 pg/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Abcam's CXCL5 Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat CXCL5 cell lysate and tissue lysate.

This assay employs an antibody specific for Rat CXCL5 coated on a 96- well plate. Standards and samples are pipetted into the wells and CXCL5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat CXCL5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CXCL5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Recovery

[ { "sample": "Cell culture extracts", "range": "82 - 102 %", "average": "= 91.25" }, { "sample": "Tissue Extracts", "range": "84 - 103 %", "average": "= 93.47" } ]
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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100775-1x96Tests", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"2X Cell Lysis Buffer", "number":"AB100775-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"CXCL5 Microplate (12 x 8 wells)", "number":"AB100775-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100775-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Sample Diluent Buffer", "number":"AB100775-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100775-CMP02", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB100775-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100775-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat CXCL5 Standard (lyophilized)", "number":"AB100775-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat CXCL5", "number":"AB100775-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"400X HRP-Streptavidin Concentrate", "number":"AB100775-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CXCL5 also known as LIX (Liposaccharide-induced CXC chemokine) is a small cytokine belonging to the CXC chemokine family. It plays a significant role in immunological responses by acting as a chemoattractant for neutrophils. CXCL5 has a molecular mass of around 12 kDa. The protein is mainly expressed in neutrophils epithelial cells and endothelial cells. It is often studied alongside its competitors in different therapeutic and diagnostic contexts.
Biological function summary

CXCL5 affects inflammation and the immune response by modulating neutrophil recruitment to sites of infection or injury. It is a part of the larger network of chemokines that direct leukocyte movement throughout the body. While CXCL5 does not form a complex in a traditional sense it does interact dynamically with CXCR2 receptor to exert its effects. These interactions are pivotal to the protein’s role in orchestrating the immune system’s response to diverse stimuli.

Pathways

CXCL5 plays a part in the chemokine signaling pathway and the inflammatory response pathway. These pathways are important in mediating immune surveillance and inflammation. CXCL5 through these pathways has a strong connection to other CXC family proteins like CXCL1 and the receptor CXCR2 which allows for a coordinated response during immune challenges and recovery processes.

CXCL5 is relevant in the context of chronic inflammatory disorders such as rheumatoid arthritis and cancer specifically its role in tumor-related inflammation. In these conditions CXCL5 is linked to the exacerbation of inflammatory states and promotion of tumor growth via angiogenesis. The protein's interactions with CXCR2 play a significant role in these disease processes representing potential therapeutic targets for managing inflammation and modulating cancer progression.
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제품 프로토콜

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타겟 정보

See full target information UGT1

대체 명칭 보기

AMCF II, C-X-C motif chemokine 5, ENA 78, Epithelial-derived neutrophil-activating protein 78, LIX, Neutrophil-activating peptide ENA-78, SCYB5, SCYB6, Small inducible cytokine subfamily B (Cys X Cys) member 5 (epithelial derived neutrophil activating peptide 78), Small inducible cytokine subfamily B, member 15, Small-inducible cytokine B5, chemokine (C-X-C motif) ligand 5, neutrophil-activating protein 78
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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