AB100760

Rat Fractalkine ELISA Kit (CX3CL1)

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Rat Fractalkine ELISA Kit (CX3CL1) is a Sandwich ELISA for the measurement of Rat Fractalkine (CX3CL1) in Rat in Biofluids, Cell Culture Media samples.

대체 명칭 보기

Acc1, Fkn, Scyd1, Cx3cl1, Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1

2 이미지

Sandwich ELISA - Rat Fractalkine ELISA Kit (CX3CL1) (AB100760)

sELISA

Supplier Data

Sandwich ELISA - Rat Fractalkine ELISA Kit (CX3CL1) (AB100760)

Representative Standard Curve using ab100760

sELISA

Supplier Data

Sandwich ELISA - Rat Fractalkine ELISA Kit (CX3CL1) (AB100760)

Representative Standard Curve using ab100760

주요 정보

검출 방식

Colorimetric

샘플 타입

Plasma, Cell culture supernatant, Serum

Reacts with

Rat

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

< 5 pg/mL

검출 범위

2.74 - 2000 pg/mL

분석 플랫폼

Microplate

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Abcam's Fractalkine Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat Fractalkine in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Rat Fractalkine coated on a 96-well plate. Standards and samples are pipetted into the wells and Fractalkine present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat Fractalkine antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Fractalkine bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

The standard is affinity purified.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 104 %", "average": "= 93.35" }, { "sample": "Serum", "range": "83 - 103 %", "average": "= 94.54" }, { "sample": "Plasma", "range": "84 - 105 %", "average": "= 93.46" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100760-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Fractalkine Microplate (12 x 8 wells)", "number":"AB100760-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100760-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100760-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100760-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100760-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100760-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Rat Fractalkine", "number":"AB100760-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Rat Fractalkine Standard (lyophilized)", "number":"AB100760-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"240X HRP-Streptavidin Concentrate", "number":"AB100760-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CX3CL1 also known as fractalkine is a chemokine protein characterized mechanically by its dual function as both a chemoattractant and adhesion molecule. It exhibits a unique membrane-bound structure and can exist in a soluble form after proteolytic cleavage. The mass of CX3CL1 is approximately 42-45 kDa. This protein is highly expressed in endothelial cells neurons and tissues with substantial vascularization. Considerable interest surrounds the potential of CX3CL1 assessment in various studies often involving applications like fractalkine ELISA or assays on mouse models.

Biological function summary

CX3CL1 is involved in the immune system and nervous system functions influencing leukocyte adhesion and migration. It is not known to be part of a large multiprotein complex but directly interacts with its fractalkine receptor CX3CR1 on the surface of immune cells. This interaction regulates communication between neurons and microglia which highlights its role in neuroinflammation. CX3CL1's expression and function suggest a significant influence over inflammatory responses and homeostatic balance within these systems.

Pathways

CX3CL1 plays a role in the MAPK and PI3K-Akt pathways vital for cell survival proliferation and migration. Within these pathways CX3CL1 influences and interacts with several proteins thereby modulating inflammatory responses and cellular migration. These pathways are essential for immune system regulation where CX3CL1 acts as a mediator in signal transduction processes that connect immune responses to cellular activities such as proliferation and survival.

CX3CL1 has associations with neurodegenerative disorders like Alzheimer's disease and inflammatory diseases such as rheumatoid arthritis. Alterations in CX3CL1 expression or its fractalkine receptor function can influence disease progression by affecting how immune cells communicate and migrate to inflamed or damaged tissues. CX3CR1 the receptor for CX3CL1 represents an important link in these connections driving research efforts to explore therapies targeting these interactions to alleviate disease symptoms.

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제품 프로토콜

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타겟 정보

Chemokine that acts as a ligand for both CX3CR1 and integrins ITGAV : ITGB3 and ITGA4 : ITGB1. The CX3CR1-CX3CL1 signaling exerts distinct functions in different tissue compartments, such as immune response, inflammation, cell adhesion and chemotaxis. Regulates leukocyte adhesion and migration processes at the endothelium. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1.. Processed fractalkine. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils.. Fractalkine. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells.

See full target information Cx3cl1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in psychiatry 7:214 PubMed28149283

2017

Plasma Chemokines in Patients with Alcohol Use Disorders: Association of CCL11 (Eotaxin-1) with Psychiatric Comorbidity.

Applications

Unspecified application

Species

Unspecified reactive species

Nuria García-Marchena,Pedro Fernando Araos,Vicente Barrios,Laura Sánchez-Marín,Julie A Chowen,María Pedraz,Estela Castilla-Ortega,Pablo Romero-Sanchiz,Guillermo Ponce,Ana L Gavito,Juan Decara,Daniel Silva,Marta Torrens,Jesús Argente,Gabriel Rubio,Antonia Serrano,Fernando Rodríguez de Fonseca,Francisco Javier Pavón

제품이 사용된 논문 모두 보기

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websiteProtocolBooklet

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Rat Fractalkine ELISA Kit (CX3CL1)

Sandwich ELISA - Rat Fractalkine ELISA Kit (CX3CL1) (AB100760)

Sandwich ELISA - Rat Fractalkine ELISA Kit (CX3CL1) (AB100760)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Secondary Antibodies

Reactivity 정보

제품 세부 정보

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise