AB309285

Rat RANKL (TNFSF11) ELISA Kit

Rat RANKL (TNFSF11) ELISA 키트

Recombinant
SimpleStep
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Rat RANKL (TNFSF11) ELISA Kit is a single-wash 90-min Simplestep used to quantify Rat RANKL (TNFSF11) with a sensitivity of 3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

대체 명칭 보기

CD254, Opgl, Rankl, Trance, Tnfsf11, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, Osteoprotegerin ligand, Receptor activator of nuclear factor kappa-B ligand, TNF-related activation-induced cytokine, ODF, OPGL, RANKL, TRANCE

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Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

sELISA

Supplier Data

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Interpolated concentrations of spiked Rat RANKL in Rat Serum, Rat Plasma (citrate), Rat Plasma (EDTA), and Rat Plasma (heparin)

Recombinant RANKL was spiked into the following Rat samples and diluted in a 2-fold dilution series in Sample Diluent 25BS. Undiluted samples are 50% Rat Plasma (citrate), Rat Plasma (EDTA), and Rat Plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

sELISA

Supplier Data

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Interpolated concentrations of Rat RANKL in Treated and Untreated Rat Splenocyte Supernatant

Interpolated concentration of native RANKL was measured in duplicate at different sample concentrations. Undiluted samples are 100% rat splenocyte treated with concanavalin A (5µg/ml, 48hr) and 100% untreated rat splenocyte supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent 25BS.

sELISA

Supplier Data

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Example of Rat RANKL standard curve in Sample Diluent 25BS. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Cell culture supernatant, EDTA Plasma, Citrate plasma, Heparin Plasma, Serum

Reacts with

Rat

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 3 pg/mL

검출 범위

23.438 - 1500 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Rat RANKL (TNFSF11) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANKL (TNFSF11) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Rat RANKL (TNFSF11) with 3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.9" } ]

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Recovery

[ { "sample": "Serum", "range": "95 - 109 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "99 - 105 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "105 - 114 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "86 - 102 %", "average": "= 93" }, { "sample": "Cell culture supernatant", "range": "100 - 119 %", "average": "= 107" } ]

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제품 구성

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309285-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309285-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309285-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB309285-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 25BS", "number":"AB309285-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309285-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309285-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309285-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Stop Solution", "number":"AB309285-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Rat RANKL (TNFSF11) Lyophilized Recombinant Protein", "number":"AB309285-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat RANKL (TNFSF11) Capture Antibody", "number":"AB309285-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Rat RANKL (TNFSF11) Detector Antibody", "number":"AB309285-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

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타겟 정보

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.

See full target information Tnfsf11

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websiteProtocolBooklet

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Product promise

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For licensing inquiries, please contact partnerships@abcam.com

Rat RANKL (TNFSF11) ELISA Kit

Rat RANKL (TNFSF11) ELISA 키트

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

Sandwich ELISA - Rat RANKL (TNFSF11) ELISA Kit (AB309285)

주요 정보

검출 방식

샘플 타입

Reacts with

분석 유형

결과 유형

Sensitivity

검출 범위

분석 소요 시간

분석 플랫폼

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

Reactivity 정보

제품 세부 정보

Precision

Recovery

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

Product promise