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AB315296

SARS-CoV2 Nucleoprotein ELISA Kit

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SARS-CoV2 Nucleoprotein ELISA Kit is a single-wash 90-min Simplestep used to quantify SARS-CoV2 Nucleoprotein with a sensitivity of 17.045 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
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Sandwich ELISA - SARS-CoV2 Nucleoprotein ELISA Kit (AB315296)
  • sELISA

Supplier Data

Sandwich ELISA - SARS-CoV2 Nucleoprotein ELISA Kit (AB315296)

Normalized signal of 3,000 pg/mL of recombinant SARS-CoV2, SARS-CoV1, or MERS-CoV nucleoprotein

3,000 pg/mL of recombinant SARS-CoV2, SARS-CoV1, or MERS-CoV nucleoprotein were tested for specificity.  Normalized OD (mean +/- SD) is graphed.  Less than 1% cross reactivity was observed for SARS-CoV1 and no cross reactivity was observed for MERS-CoV nucleoprotein.

Sandwich ELISA - SARS-CoV2 Nucleoprotein ELISA Kit (AB315296)
  • sELISA

Supplier Data

Sandwich ELISA - SARS-CoV2 Nucleoprotein ELISA Kit (AB315296)

Interpolated concentrations of spiked human SARS-CoV2 Nucleoprotein in human serum, plasma (EDTA), saliva, and cell culture media

Recombinant SARS-CoV2 Nucleoprotein was spiked into the following human samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are as follows : serum 25%, plasma (EDTA) 25%, saliva 50%, and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - SARS-CoV2 Nucleoprotein ELISA Kit (AB315296)
  • sELISA

Supplier Data

Sandwich ELISA - SARS-CoV2 Nucleoprotein ELISA Kit (AB315296)

Example of human SARS-CoV2 Nucleoprotein standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

검출 방식

Colorimetric

샘플 타입

Serum, EDTA Plasma, Saliva, Cell culture media

Reacts with

SARS-CoV-2

분석 유형

Sandwich

결과 유형

Quantitative

Sensitivity

= 17.045 pg/mL

검출 범위

46.875 - 3000 pg/mL

분석 소요 시간

1h 30m

분석 플랫폼

Pre-coated microplate (12 x 8 well strips)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

SARS-CoV2 Nucleoprotein SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SARS-CoV2 Nucleoprotein protein in human serum, plasma, saliva and cell culture supernatant. Quantitate  SARS-CoV2 Nucleoprotein with 17.045 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Cell culture media", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.5" }, { "reproducibilityType": "Inter", "sample": "Cell culture media", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 1.4" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 97 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "73 - 77 %", "average": "= 76" }, { "sample": "Saliva", "range": "103 - 113 %", "average": "= 109" }, { "sample": "Cell culture media", "range": "89 - 93 %", "average": "= 91" } ]
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제품 구성

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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The nucleocapsid protein of SARS-CoV-2 also known as N protein or COVID-19 nucleocapsid protein is important for the viral replication and packaging processes. It has a mass of approximately 43-46 kDa and is expressed in the cytoplasm of infected host cells. This protein is highly conserved among coronaviruses and plays a significant role in the viral lifecycle by binding to the viral RNA genome thereby facilitating its encapsulation within the virion. Moreover it aids in stabilizing the viral RNA structure and is essential for ribonucleoprotein complex formation.
Biological function summary

The nucleocapsid protein of SARS-CoV-2 interacts directly with other viral components and host cellular machinery to orchestrate efficient viral assembly and release. It forms a ribonucleoprotein complex by self-association and binding to the RNA genome promoting virion integrity. This protein also plays a part in modulating host cell responses potentially by interfering with antiviral signaling pathways ensuring that the virus evades the host immune system to some degree.

Pathways

Many scientific studies show that the SARS-CoV-2 nucleocapsid protein is involved in the viral replication and assembly pathways. It interacts with viral proteins such as the membrane (M) protein and possibly others to facilitate these processes. The protein also exhibits connectivity with cellular pathways related to RNA processing and metabolism due to its RNA-binding capabilities further influencing viral replication efficiency and host-pathogen interactions.

The nucleocapsid protein has a significant association with COVID-19 the disease caused by SARS-CoV-2. Its expression correlates with viral pathogenicity and immune response dysregulation which can lead to severe outcomes in infected individuals. The N protein has links with comorbid conditions potentially affecting disease progression. Furthermore the nucleocapsid protein interacts with host proteins like G3BP1 and other cellular factors that may contribute to immune modulation influencing the clinical symptoms associated with COVID-19.
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제품 프로토콜

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타겟 정보

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M (PubMed : 33264373). Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. Attenuates the stress granules formation by reducing host G3BP1 access to host mRNAs under stress conditions (PubMed : 34901782, PubMed : 36534661).. May block host chemokine function in vivo, facilitating viral replication and transmission (PubMed : 35921414). Acts by being secreted into the extracellular space where it competes to host chemokines for binding to host glycosaminoglycans (GAG) (PubMed : 35921414).. May induce inflammasome responses in cultured cells and mice. Acts by interacting with host NLRP3 to facilitate inflammasome assembly, which induces cytokine release that may play a role in COVID lung injury. Disrupts the association betwen NLRP3 and ABHD8, enhancing NLRP3 stability and leading to increased inflammasome activation (PubMed : 39225180).
See full target information N

대체 명칭 보기

Nucleoprotein, N, Nucleocapsid protein, NC, Protein N
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websiteProtocolBooklet
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Product promise

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