3D Cell Culture Clearing Kit - hydrophobic

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3D Cell Culture Clearing Kit - hydrophobic ab243299 enables the easy set-up of rapid 3D cell culture clearing for 3D imaging using simple protocols and standard laboratory equipment.

This kit can be used with immunostaining, fluorescent proteins and chemical dyes. Clearing is reversible so that you can treat your sample for conventional H&E or IHC staining after 3D imaging.

To help you get started with clearing 3D cell cultures, we have clearing validated antibodies. Validated antibodies are for key markers, such as NeuN, GFAP, Iba1, etc, and are tested for tissue clearing with 1 mm mouse brain sections, and for 3D cell culture clearing with neuronal spheroids and hepatic spheroids (depending on the marker).

If you want to set up clearing of 3D cell cultures on a sample type that we haven’t tested or to use your own antibodies, please consult the protocol book for this kit.

To learn more about tissue clearing and clearing of 3D cell cultures, please review our guide to tissue clearing and clearing 3D cell cultures.

Clearing reagents and companion products are also available as individual products.

These clearing reagents were developed with Visikol Inc, who developed the Visikol® HISTO™ clearing technology which is the basis of our reagents.