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AB309564

Human DNase I Antibody Pair - BSA and Azide free

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Human DNase I Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human DNase I.

대체 명칭 보기

DNL1, DRNI, DNASE1, Deoxyribonuclease-1, Deoxyribonuclease I, DNase I

1 이미지
Sandwich ELISA - Human DNase I Antibody Pair - BSA and Azide free (AB309564)
  • sELISA

Supplier Data

Sandwich ELISA - Human DNase I Antibody Pair - BSA and Azide free (AB309564)

Sandwich ELISA of ab309564 with the capture antibody dilution at 2 μg/mL and detector antibody dilution at 0.5 μg/mL.

주요 정보

Reacts with

Human

분석 유형

ELISA set

검출 범위

3.9 - 500 pg/mL

분석 플랫폼

Reagents

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보완 제품

Assay Kits

AB234056

DNase I Assay Kit (Fluorometric)

Functional Studies - DNase I Assay Kit (Fluorometric) (AB234056)

  • 4
  • 1
Proteins & Peptides

AB275555

Recombinant human DNase I protein (Active)

Functional Studies - Recombinant human DNase I protein (Active) (AB275555)

  • 0
  • 0
Assay Kits

AB315786

Human DNase I ELISA Kit

SimpleStep

Sandwich ELISA - Human DNase I ELISA Kit (AB315786)

  • 0
  • 0
Primary Antibodies

AB309566

Anti-DNase I antibody [EPR25941-19] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-DNase I antibody [EPR25941-19] - BSA and Azide free (Detector) (AB309566)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human DNase I Antibody Pair - BSA and Azide free (ab309564) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify human DNase I in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab309564-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human DNase I Detector Antibody (unconjugated)", "number":"AB309564-CMP02", "productcode":"AB309566" }, { "size":"1 x 100 µg", "name":"Human DNase I Capture Antibody (unconjugated)", "number":"AB309564-CMP01", "productcode":"AB309565" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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제품 프로토콜

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타겟 정보

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed : 11241278, PubMed : 2251263, PubMed : 2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (By similarity). Among other functions, seems to be involved in cell death by apoptosis (PubMed : 11241278). Binds specifically to G-actin and blocks actin polymerization (By similarity). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity).
See full target information DNASE1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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