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AB220136

Human IRAK-1 Matched Antibody Pair Kit

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Human IRAK-1 Matched Antibody Pair Kit is a kit for ELISA development for the measurement of Human IRAK-1 by sandwich ELISA method.
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ELISA - Human IRAK-1 Matched Antibody Pair Kit (AB220136)
  • ELISA

Supplier Data

ELISA - Human IRAK-1 Matched Antibody Pair Kit (AB220136)

Human IRAK-1 standard curve

주요 정보

검출 방식

Colorimetric

Reacts with

Human

분석 유형

ELISA set

Sensitivity

= 2.5 pg/mL

검출 범위

62.5 - 4000 pg/mL

분석 플랫폼

Reagents

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보완 제품

Assay Kits

AB218182

Human IRAK4 Matched Antibody Pair Kit

Recombinant

ELISA - Human IRAK4 Matched Antibody Pair Kit (AB218182)

  • 0
  • 0
Assay Kits

AB210905

ELISA Accessory Pack

  • 0
  • 0
Proteins & Peptides

AB79739

Recombinant human IRAK4 protein (GST tag N-Terminus)

Functional Studies - Recombinant human IRAK4 protein (GST tag N-Terminus) (AB79739)

  • 0
  • 0
Assay Kits

AB215402

Human IL-8 Matched Antibody Pair Kit

Recombinant

ELISA - Human IL-8 Matched Antibody Pair Kit (AB215402)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human IRAK-1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IRAK-1.Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our . An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219630. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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제품 구성

{ "values": { "5x96Tests": { "sellingSize": "5 x 96 Tests", "publicAssetCode":"ab220136-5x96Tests", "assetComponentDetails": [ { "size":"1 x 12.5 µg", "name":"Human IRAK-1 Detector Antibody", "number":"AB220136-CMP02", "productcode":"" }, { "size":"1 x 50 µg", "name":"Human IRAK-1 Capture Antibody", "number":"AB220136-CMP01", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Human IRAK-1 Lyophilized Protein", "number":"AB220136-CMP03", "productcode":"" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

IRAK-1 also known as Interleukin-1 Receptor-Associated Kinase 1 is a serine/threonine kinase that plays a role in the immune response. With a molecular mass around 76 kDa this protein is heavily expressed in immune cells such as macrophages and monocytes. IRAK-1 is an important player in the signal transduction process following the activation of the Toll-like and interleukin-1 receptor (IL-1R) families. The kinase activity of IRAK-1 occurs when it associates with the receptor complex transmitting signals that activate downstream pathways.
Biological function summary

IRAK-1 mediates the inflammatory response by promoting the production of cytokines and other inflammatory mediators. It is a component of the Myddosome a multi-protein complex that assembles upon receptor activation and includes MyD88 an adaptor protein needed for signal transduction. IRAK-1 phosphorylates other targets and itself leading to its own degradation which is an important step for turning off the signaling and preventing prolonged activation. Its precise activity modulation ensures balanced immune responses and prevents overreaction that could cause tissue damage.

Pathways

IRAK-1 functions in the IL-1 and Toll-like receptor signaling pathways both of which are important for innate immunity. In these pathways IRAK-1 interacts with key proteins such as TRAF6 which transduces signals leading to NF-κB and MAPK activation critical for inflammatory gene expression. Its role in these pathways highlights the importance of proper function and regulation as deficiencies or dysregulation can result in inappropriate immune responses.

IRAK-1 has been connected to autoimmune diseases such as rheumatoid arthritis and certain cancers. This protein affects how the immune system responds to inflammation and can influence disease progression through its regulatory actions. For instance alterations in IRAK-1 activity or expression can lead to an increase in pro-inflammatory cytokines that exacerbate conditions like rheumatoid arthritis. Additionally in cancer pathways influenced by IRAK-1 can affect tumor growth and metastasis. Its interaction with proteins such as MyD88 and TRAF6 within these pathological contexts underlines its importance as a potential target for therapeutic intervention.
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제품 프로토콜

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타겟 정보

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
See full target information IRAK1

대체 명칭 보기

IRAK, IRAK1, Interleukin-1 receptor-associated kinase 1, IRAK-1
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websiteProtocolBooklet
en
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Product promise

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