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AB288642

Human LAMP2 Antibody Pair - BSA and Azide free

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Human LAMP2 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human LAMP2.

대체 명칭 보기

CD107b, Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, LGP-96, LAMP2

2 이미지
Sandwich ELISA - Human LAMP2 Antibody Pair - BSA and Azide free (AB288642)
  • sELISA

Supplier Data

Sandwich ELISA - Human LAMP2 Antibody Pair - BSA and Azide free (AB288642)

Example of human LAMP2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human LAMP2 Antibody Pair - BSA and Azide free (AB288642)
  • sELISA

Supplier Data

Sandwich ELISA - Human LAMP2 Antibody Pair - BSA and Azide free (AB288642)

Example of Human LAMP2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

Reacts with

Human

분석 유형

ELISA set

분석 플랫폼

Reagents

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보완 제품

Primary Antibodies

AB125068

Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker

20ul selling size|RabMAb|Recombinant|Lab Essentials|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2A antibody [EPR4207(2)] - Lysosome Marker (AB125068)

  • 4
  • 4
Assay Kits

AB302757

Human LAMP2 ELISA Kit

Recombinant|SimpleStep

ELISA - Human LAMP2 ELISA Kit (AB302757)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Human LAMP2 Antibody Pair - BSA and Azide free (ab288642) is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human LAMP2 in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab288642-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human LAMP2 Detector Antibody (unconjugated)", "number":"AB288642-CMP01", "productcode":"AB288624" }, { "size":"1 x 100 µg", "name":"Human LAMP2 Capture Antibody (unconjugated)", "number":"AB288642-CMP02", "productcode":"AB288606" } ] } } }
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특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

LAMP2 also known as CD107b is an important protein found in the lysosomal membrane. It plays a significant role in lysosome function facilitating autophagy and the degradation of cellular debris. LAMP2 proteins are expressed in most tissues especially in tissues with high lysosomal activity like the liver kidney and heart. The LAMP2 molecule has a molecular weight of approximately 45 kDa. Researchers frequently use LAMP2 staining and LAMP2 marker to study its cellular localization and expression levels.
Biological function summary

LAMP2 is essential for the normal fusion of lysosomes with autophagosomes. This process ensures the recycling of cellular components and maintenance of cellular homeostasis. LAMP2 acts in conjunction with other members of the lysosome-associated membrane protein family LAMP1 and LAMP3 forming part of a larger complex. This interaction is fundamental in managing the degradation pathways within the cell supporting processes such as lipid metabolism and protein turnover.

Pathways

The activity of LAMP2 is critical in the autophagic and degradation pathways within the cell. It particularly interacts in the autophagy-lysosome pathway where it aids the clearance of damaged organelles and protein aggregates. LAMP2 interaction with proteins such as the autophagy-related protein LC3 helps this degradation process. Additionally LAMP2 plays a role in endocytic pathways linking it to proteins involved in vesicle trafficking and membrane fusion.

Mutations or deficiencies in LAMP2 are associated with Danon disease a type of lysosomal storage disorder. Patients with Danon disease exhibit symptoms such as cardiomyopathy skeletal myopathy and intellectual disabilities. LAMP2 is also implicated in other lysosomal disorders with a connection to the malfunction of autophagy processes. Its dysfunction is often linked with proteins responsible for cellular degradation affecting the normal homeostatic balance and leading to the accumulation of autophagic substrates.
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제품 프로토콜

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타겟 정보

Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation and autophagy (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 27628032, PubMed : 36586411, PubMed : 37390818, PubMed : 8662539). Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity (PubMed : 37390818). Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 27628032, PubMed : 36586411, PubMed : 8662539). Functions by binding target proteins, such as GAPDH, GPX4, NLRP3 and MLLT11, and targeting them for lysosomal degradation (PubMed : 11082038, PubMed : 18644871, PubMed : 24880125, PubMed : 36586411, PubMed : 8662539). In the chaperone-mediated autophagy, acts downstream of chaperones, such as HSPA8/HSC70, which recognize and bind substrate proteins and mediate their recruitment to lysosomes, where target proteins bind LAMP2 (PubMed : 36586411). Plays a role in lysosomal protein degradation in response to starvation (By similarity). Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed : 27628032). Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed : 27628032). Required for normal degradation of the contents of autophagosomes (PubMed : 27628032). Required for efficient MHC class II-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHC II subunits (PubMed : 15894275, PubMed : 20518820). Is not required for efficient MHC class II-mediated presentation of endogenous antigens (PubMed : 20518820).. Isoform LAMP-2C. Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
See full target information LAMP2
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Product promise

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