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AB244094

Human/Mouse GFAP Antibody Pair - BSA and Azide free

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Human/Mouse GFAP Antibody Pair - BSA and Azide free is a matched antibody pair validated for use in sandwich-based immunoassays.

- Contains a capture and a detector antibody at a concentration of approx. 1mg/mL.
- Carrier free (PBS only) - formulation: ideal for optimal conjugation to your label of choice
- Antibody orientation based on internal optimization for ELISA-based assays
- Capture and detector antibodies also available to purchase individually.

대체 명칭 보기

Glial fibrillary acidic protein, GFAP, Gfap

1 이미지
Sandwich ELISA - Human/Mouse GFAP Antibody Pair - BSA and Azide free (AB244094)
  • sELISA

Supplier Data

Sandwich ELISA - Human/Mouse GFAP Antibody Pair - BSA and Azide free (AB244094)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab233621), which uses the same antibody pair. For additional information on the performance of pair and kit, refer to the corresponding kit datasheet. Due to differences in the formulation and format of the antibodies in this pair, they cannot be used as substitutes for the antibody components in our SimpleStep ELISA® Kits.

주요 정보

Reacts with

Mouse, Human

분석 유형

ELISA set

검출 범위

125 - 8000 pg/mL

분석 플랫폼

Reagents

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

제품 세부 정보

This Antibody Pair can be used to quantify Human/Mouse GFAP. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.

For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA®Kit (ab233621), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.

Patented technology

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery® (MSD®)
Bead-based assays
AlphaLISA®/AlphaScreen®
DELFIA® immunoassays
Simoa® and Single Molecule Counting (SMC) immunoassays
Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: (also available as ab242692)

recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody:(also available as ab242937)

recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration

: approx. 1 mg/ml

Storage buffer:

100% PBS

Form:

Liquid

Isotype:

IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab244094-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human/Mouse GFAP Detector Antibody (unconjugated)", "number":"AB244094-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human/Mouse GFAP Capture Antibody (unconjugated)", "number":"AB244094-CMP01", "productcode":"" } ] } } }

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C

제품 프로토콜

타겟 정보

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
See full target information Gfap

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Alzheimer's research & therapy 13:58 PubMed33678186

2021

Development of a novel, sensitive translational immunoassay to detect plasma glial fibrillary acidic protein (GFAP) after murine traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Emily B Button,Wai Hang Cheng,Carlos Barron,Honor Cheung,Asma Bashir,Jennifer Cooper,Jasmine Gill,Sophie Stukas,David C Baron,Jerome Robert,Elyn M Rowe,Peter A Cripton,Cheryl L Wellington
제품이 사용된 논문 모두 보기

Product promise

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