AB253655

Mouse TREM2 Antibody Pair - BSA and Azide free

RabMAb
Recombinant
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Mouse TREM2 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Mouse TREM2.

대체 명칭 보기

Trem2a, Trem2b, Trem2c, Trem2, Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2

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Sandwich ELISA - Mouse TREM2 Antibody Pair - BSA and Azide free (AB253655)

sELISA

Supplier Data

Sandwich ELISA - Mouse TREM2 Antibody Pair - BSA and Azide free (AB253655)

Example of mouse TREM2 standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

Reacts with

Mouse

분석 유형

ELISA set

검출 범위

7.8 - 1000 pg/mL

분석 플랫폼

Reagents

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Mouse TREM2 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Mouse TREM2 in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery^® (MSD^®)
Bead-based assays
AlphaLISA^®/AlphaScreen^®
DELFIA^® immunoassays
Simoa^® and Single Molecule Counting (SMC™) immunoassays
Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

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제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab253655-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Mouse TREM2 Detector Antibody (unconjugated)", "number":"AB253655-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Mouse TREM2 Capture Antibody (unconjugated)", "number":"AB253655-CMP01", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

+4°C

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed : 11241283). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed : 27477018, PubMed : 29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (PubMed : 27477018, PubMed : 29518356). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed : 27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (By similarity). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (PubMed : 28077724). Required for microglial phagocytosis of apoptotic neurons (PubMed : 24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (PubMed : 15728241, PubMed : 25631124, PubMed : 28592261, PubMed : 29752066). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (PubMed : 28483841, PubMed : 29663649, PubMed : 29859094, PubMed : 30232263). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (PubMed : 29663649). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (PubMed : 28592261). Plays a role in microglial MTOR activation and metabolism (PubMed : 28802038). Regulates age-related changes in microglial numbers (PubMed : 25631124, PubMed : 29752066, PubMed : 30548312). Triggers activation of the immune responses in macrophages and dendritic cells. Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed : 18957693). In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed : 16715077). Involved in the positive regulation of osteoclast differentiation (PubMed : 16418779).

See full target information Trem2

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mouse TREM2 Antibody Pair - BSA and Azide free

Sandwich ELISA - Mouse TREM2 Antibody Pair - BSA and Azide free (AB253655)

주요 정보

Reacts with

분석 유형

검출 범위

분석 플랫폼

보완 제품

Assay Kits

Primary Antibodies

Assay Kits

Proteins & Peptides

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

Product promise