JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB253705

Rat Ghrelin active Antibody Pair - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rat Ghrelin active Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Rat Ghrelin active.

대체 명칭 보기

Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Ghrl

1 이미지
Sandwich ELISA - Rat Ghrelin active Antibody Pair - BSA and Azide free (AB253705)
  • sELISA

Lab

Sandwich ELISA - Rat Ghrelin active Antibody Pair - BSA and Azide free (AB253705)

Sandwich ELISA of ab253705 with the capture antibody ab281265 dilution at 2 µg/mL and detector antibody ab281115 dilution at 0.5 µg/mL.

주요 정보

Reacts with

Rat

분석 유형

ELISA set

검출 범위

7.81 - 500 pg/mL

분석 플랫폼

Reagents

style
left-column

보완 제품

Assay Kits

AB256762

Human PYY Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human PYY Antibody Pair - BSA and Azide free (AB256762)

  • 0
  • 0
Assay Kits

AB307310

Human GIP Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human GIP Antibody Pair - BSA and Azide free (AB307310)

  • 0
  • 0
Assay Kits

AB179884

Human Leptin ELISA Kit

SimpleStep

ELISA - Human Leptin ELISA Kit (AB179884)

  • 0
  • 0
Assay Kits

AB239421

Rat Adiponectin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Rat Adiponectin ELISA Kit (AB239421)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Rat GHRL Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Rat GHRL in sandwich ELISAs and many other pair-based applications.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The pair can be used in variety of assays and platforms including but not limited to:

  • Sandwich ELISA
  • FRET/TR-FRET/HTRF
  • Meso Scale Discovery® (MSD®)
  • Bead-based assays
  • AlphaLISA®/AlphaScreen®
  • DELFIA® immunoassays
  • Simoa® and Single Molecule Counting (SMC™) immunoassays
  • Multiplex

Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:

  • Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
  • Concentration of ~1 mg/ml as measured by the protein A280 method.

We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.

Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.

Please note:

The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.

The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.

We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.

Antibody properties:

Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg

Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg

Concentration: ~1 mg/ml

Storage buffer: 100% PBS

Form: Liquid

Isotype: IgG

Recombinant monoclonal antibodies offer several advantages including:

- High batch-to-batch consistency and reproducibility

- Improved sensitivity and specificity

- Long-term security of supply

- Animal-free production

Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.

AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.

Simoa is a registered trademark of Quanterix, Inc.

SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

style
left-column

제품 구성

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab253705-10x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Rat GHRL active Detector Antibody (unconjugated)", "number":"AB253705-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Rat GHRL active Capture Antibody (unconjugated)", "number":"AB253705-CMP02", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Ghrelin also known as the "hunger hormone" or GH secretagogue is a peptide hormone with a mass of 28 amino acids. Ghrelin is mainly expressed in the stomach particularly in the enteroendocrine cells but it can also be found in small amounts in the lungs pancreas and hypothalamus. The hormone increases food intake and promotes fat storage by signaling the brain to stimulate hunger. Its secretion occurs in response to fasting and is suppressed by food intake reflecting its role in energy balance.
Biological function summary

Ghrelin influences a variety of physiological processes beyond hunger regulation. It plays a role in modulating the release of growth hormone by acting on the growth hormone secretagogue receptor (GHS-R). Ghrelin also affects gastric motility and acid secretion due to its expression in the gastrointestinal tract. Though not part of a larger complex its interaction with GHS-R strongly influences its biological functions. Ghrelin may also impact insulin secretion and sensitivity linking it to glucose metabolism processes.

Pathways

Ghrelin interacts with several critical biological networks. Ghrelin is involved in the neuroendocrine pathway that connects the gut to the brain and contributes significantly to energy homeostasis. Within this pathway it influences proteins like leptin which exerts opposite effects by suppressing hunger. Additionally ghrelin functions in the hedonic control of eating which relates to the rewarding aspects of food intake controlled by pathways involving dopamine.

Ghrelin holds relevance to obesity and cachexia a form of extreme weight loss often seen in chronic illness. Ghrelin promotes adiposity by stimulating appetite which can contribute to obesity. Conversely in cachexia the decrease in ghrelin activity is thought to exacerbate weight loss. Its role in energy balance associates ghrelin with the protein leptin which counterbalances its effects and connections to metabolic disorders. Understanding ghrelin’s functions and pathways provides insight into therapeutic strategies for these conditions.
style
left-column

제품 프로토콜

style
left-column

타겟 정보

Precursor of the appetite-regulating peptide ghrelin, including ghrelin-27 and ghrelin-28.. Ghrelin. Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR) (By similarity). Octanoylated ghrelin-28 is the most commonly found ghrelin and octanoylation is essential for the activation of the growth hormone secretagogue receptor type 1 (GHSR) (By similarity). Induces the release of growth hormone from the pituitary (By similarity). Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.. Obestatin-23. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
See full target information Ghrl
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com