MitoBiogenesis™ Western Blot Cocktail

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The MitoBiogenesis™ western blotting cocktail (ab123545) is designed to study the regulation of mitochondrial biogenesis and cellular stress in response to environmental stimuli. It also can be used to monitor drug-induced effects on mitochondrial biogenesis. The two main components of this cocktail target two proteins, which are each subunits of a different oxidative phosphorylation enzyme complex, one subunit I of Complex IV (COX-I), which is mtDNA-encoded, and the 70kDa subunit of Complex II (SDH-A), which is nDNA-encoded. Complex IV includes several proteins which are encoded in the mitochondrion, while the proteins of Complex II are entirely encoded in the nucleus. An anti-Beta actin antibody is used as loading control.

Cocktail Antibodies:

Mouse CII-70 (SDHA) [2E3GC12FB2AE2] monoclonal:

Amount: 25 μg

Working concentration: 0.5 μg/ml

Mouse CIV-1 (MT-CO1) [1D6E1A8] monoclonal:

Amount: 50 μg

Working concentration: 1 μg/ml

Mouse Beta actin [mAbcam 8224] monoclonal:

Amount: 25 μg

Working concentration: 0.5 μg/ml

Antibody Purity: Near homogeneity as judged by SDS-PAGE. The antibodies were produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

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Review the mitochondrial assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.