Anti-14-3-3 zeta antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-14-3-3 zeta antibody (AB51129)

ICC/IF image of ab51129 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51129, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (AB51129)

This image shows paraffin-embedded human breast carcinoma tissue stained with ab51129 at a dilution of 1/100. Right hand image : tissue treated with immunising peptide; left hand image : untreated tissue

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-14-3-3 zeta antibody (AB51129)

ab51129 staining 14-3-3 zeta in Human glioblastoma samples (A) and low grade tumors (B) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) overnight. Nuclei stained blue with hematoxylin. Scale bars = 20 μm

Simeone P et al., PLoS One 9:e103030 (2014), Fig 7., doi: 10.1371/journal.pone.0103030 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Unknown

Western blot - Anti-14-3-3 zeta antibody (AB51129)

All lanes:

Western blot - Anti-14-3-3 zeta antibody (ab51129)

Lane 1:

Extracts from 293 cells treated with Forskolin (40nM, 30min) with no peptide

Lane 2:

Extracts from 293 cells treated with Forskolin (40nM, 30min) with immunising peptide

Predicted band size: 28 kDa

Observed band size: 28 kDa

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