AB7794

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker

Name: Anti-160 kD Neurofilament Medium 항체 [NF-09] - Neuronal Marker (ab7794) | Abcam
Brand: Abcam Limited
SKU: AB7794
Rating: 4 (5 reviews)

Anti-160 kD Neurofilament Medium 항체 [NF-09] - Neuronal Marker

KO Validated
Lab Essentials
각 태그의 뜻

(5 리뷰들)

(67 제품이 사용된 논문 )

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody detecting 160 kD Neurofilament Medium in Western Blot, IHC-P, IHC-Fr, ICC/IF, ELISA. Suitable for Cat, Cow, Human, Mouse, Pig, Rat.

- KO validated for confirmed specificity
- Over 50 publications
- Trusted since 2002

대체 명칭 보기

NEF3, NFM, NEFM, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein

5 이미지

Lab

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Lanes 1 - 4 : Merged signal (red and green). Green - ab7794 observed at 150 kDa. Red - loading control, ab52866, observed at 50 kDa.

ab7794 was shown to specifically react with NEFM (Neurofilament) in wild-type HEK-293 cells as signal was lost in NEFM knockout cells. Wild-type and NEFM knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab7794 and ab52866 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 whole cell lysate at 20 µg

Lane 2:

NEFM knockout HEK-293 whole cell lysate at 20 µg

Lane 3:

A549 whole cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 150 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunofluorescence analysis of 1 month neuronal-differentiated dental pulp stem cells, staining 160 kD Neurofilament Medium with ab7794.

Cells were fixed with paraformaldehyde and incubated with primary antibody (1/600). A FITC-conjugated anti-mouse IgG (1/375) was used as the secondary antibody.

Image from Ferro F et al., PLoS One. 2012;7(7):e41774. Epub 2012 Jul 23. Fig 3.; doi:10.1371/journal.pone.0041774; July 23, 2012, PLoS ONE 7(7): e41774.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

ab7794 staining Neurofilament medium protein in mouse Neuro2A cells by ICC/IF.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

ICC/IF image of ab7794 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7794, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Supplier Data

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.

All lanes:

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794)

Predicted band size: 102 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

NF-09

Isotype

IgG2a

Light chain 타입

unknown

Carrier free

Reacts with

Mouse, Rat, Human, Pig, Cow, Cat

Applications

IHC-P, ICC/IF, WB, ELISA, IHC-Fr, IHC (PFA fixed)

applications

Specificity

This antibody reacts with both phosphorylated and non-phosphorylated forms of medium neurofilament protein (160 kDa) of various species.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCPFAfixed" : {"fullname" : "Immunohistochemistry (PFA fixed)", "shortname":"IHC (PFA fixed)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Cat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Mammals": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "predicted", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" }, "Pig": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCPFAfixed-species-checked": "guaranteed", "IHCPFAfixed-species-dilution-info": "", "IHCPFAfixed-species-notes": "" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Cat, Cow, Human, Mouse, Pig, Rat samples.

Trusted by the scientific community
Anti-160 kD Neurofilament Medium [NF-09] - Neuronal Marker (ab7794) was first used in a scientific publication in 2002 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-160 kD Neurofilament Medium [NF-09] - Neuronal Marker (ab7794) has over 5 independent reviews from customers.

Specificity confirmed
The specificity of Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (ab7794) has been confirmed by Western blot testing in NEFM Knockout HEK-293 cells.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The 160 kD Neurofilament Medium also known as NEFM NF-M or NEF-M belongs to the family of neurofilament proteins. This protein is an important structural component of neurons particularly in the axonal cytoskeleton. Neurofilament Medium has a molecular mass of around 160 kilodaltons (kD). It shows high levels of expression in neuronal tissues contributing to the maintenance of axonal caliber. Commonly research recognizes it alongside other neurofilament proteins emphasizing its role in providing structural integrity to neurons.

Biological function summary

Neurofilament Medium participates in the formation of a stable network of neurofilaments within neurons. Acting as a part of the intermediate filament protein family it forms a complex with other neurofilaments such as NF-L and NF-H. This complex supports neuron structure and plays an important role in axonal transport. The heteropolymeric nature of neurofilaments contributes to their mechanical stability facilitating essential neuronal functions by aligning neurofilaments along the axon.

Pathways

Neurofilament Medium integrates into the cytoskeletal arrangement pathways that govern axonal transport and stability. It plays a significant role in the neuronal transport pathway associating with other proteins such as dynein and kinesin responsible for the motor functions along axons. Also it relates to the MAP kinase pathway where phosphorylation events modulate its assembly and disassembly dynamics in response to cellular signals.

Neurofilament Medium closely relates to neurodegenerative conditions such as amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. Aberrant phosphorylation or aggregation of NEFM can disrupt neuronal function leading to pathology. In ALS the abnormal accumulation of neurofilaments correlates with motor neuron degeneration. In Alzheimer's its interaction with tau protein poses significant interest as altered states of either could exacerbate neurofibrillary tangles worsening cognitive decline.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Neurofilaments usually contain three intermediate filament proteins : NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).

See full target information NEFM

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제품이 사용된 논문 (67)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature 646:1232-1242 PubMed40931074

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Neuronal activity-dependent mechanisms of small cell lung cancer pathogenesis.

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Applications

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Communications biology 7:1713 PubMed39738805

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Abnormal cytoskeletal remodeling but normal neuronal excitability in a mouse model of the recurrent developmental and epileptic encephalopathy-susceptibility KCNB1-p.R312H variant.

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Mohammed Athamneh,Nassam Daya,Andreas Hentschel,Andrea Gangfuss,Tobias Ruck,Adela Della Marina,Ulrike Schara-Schmidt,Albert Sickmann,Anne-Katrin Güttsches,Marcus Deschauer,Corinna Preusse,Matthias Vorgerd,Andreas Roos

Science translational medicine 16:eadf4601 PubMed38446899

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Applications

Unspecified application

Species

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TaeHyun Kim,Benjamin T James,Martin C Kahn,Cristina Blanco-Duque,Fatema Abdurrob,Md Rezaul Islam,Nicolas S Lavoie,Manolis Kellis,Li-Huei Tsai

Frontiers in immunology 15:1347948 PubMed38370417

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IgLON5 deficiency produces behavioral alterations in a knockout mouse model.

Applications

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Species

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제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker

Anti-160 kD Neurofilament Medium 항체 [NF-09] - Neuronal Marker

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Immunocytochemistry/ Immunofluorescence - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

Western blot - Anti-160 kD Neurofilament Medium antibody [NF-09] - Neuronal Marker (AB7794)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (67)

Product promise