Anti-2B4 antibody [EPR30263-524]

• Flow Cyt

Lab

Flow Cytometry - Anti-2B4 antibody [EPR30263-524] (AB325752)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T cells transfected with a mouse Cd244 expression vector containing a myc-His-tag® (Middle) 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling 2B4 with ab325752 at 1/50000 dilution (0.001ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control or our antibody. Then fixed with 4% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

• Flow Cyt

Lab

Flow Cytometry - Anti-2B4 antibody [EPR30263-524] (AB325752)

Flow cytometric analysis of Mouse spleen cell cells labelling 2B4 with ab325752 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor®488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-NK1.1 conjugated to Brilliant Violet 421.

• Flow Cyt

Lab

Flow Cytometry - Anti-2B4 antibody [EPR30263-524] (AB325752)

Flow cytometric analysis of Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling 2B4 with ab325752 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor®488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD14 conjugated to Brilliant Violet 421.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-2B4 antibody [EPR30263-524] (AB325752)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC cells labelling 2B4 with ab325752 at 1/50 (9.88 µg/ml) dilution , followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing membranous and cytoplasmic staining in subsets of mouse PBMCs(shown in green), which is not co-stain with CD45R marker. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-human/mouse CD45R/B220 rat monoclonal antibody (Alexa Fluor®647) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor®488) preadsorbed at 1/1000 dilution.

• Flow Cyt

Lab

Flow Cytometry - Anti-2B4 antibody [EPR30263-524] (AB325752)

Flow cytometric analysis of Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling 2B4 with ab325752 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor®488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD45R/B220 conjugated to Alexa Fluor®647.

• Flow Cyt

Lab

Flow Cytometry - Anti-2B4 antibody [EPR30263-524] (AB325752)

Flow cytometric analysis of Mouse spleen cell cells labelling 2B4 with ab325752 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor®488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD19 conjugated to Alexa Fluor®647.