Anti-53BP1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (AB21083)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for 53BP1 protein at nucleus with ab21083 at a 1/1000 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (AB21083)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 53BP1 with ab21083 at 1/4000 dilution. Nuclear staining is observed. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (AB21083)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling 53BP1 with ab21083 at 1/4000 dilution. Nuclear staining is observed. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-53BP1 antibody (AB21083)

Immunofluorescence analysis of HeLa cells fixed in ice-cold MeOH for 5 min labelling 53BP1 protein at nucleus using ab21083 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by an anti-alpha Tubulin antibody at a 1/500 dilution (Red).
Scale bar= 10 µm.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 antibody (AB21083)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue stained for 53BP1 protein at nucleus with ab21083 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

• WB

Supplier Data

Western blot - Anti-53BP1 antibody (AB21083)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 15840649).

In Western blot, anti-53BP1 antibody (ab320755, left) and (ab21083, right) were used at 1/1000.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-53BP1 antibody [RM1222] (<a href='/ko/products/primary-antibodies/53bp1-antibody-rm1222-ab320755'>ab320755</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 40 µg

Lane 2:

Human testis tissue lysate at 40 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse placenta tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 350 kDa,124 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-53BP1 antibody (AB21083)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 15840649).

In Western blot, anti-53BP1 antibody (ab320755, left) and (ab21083, right) were used at 1/1000. A band was observed at 350 kDa in wild-type HAP1 cell lysates whereas no signal was observed at this size in the 53BP1 knockout cell line.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-53BP1 antibody [RM1222] (<a href='/ko/products/primary-antibodies/53bp1-antibody-rm1222-ab320755'>ab320755</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

53BP1 knockout HAP1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 350 kDa,124 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-53BP1 antibody (AB21083)

5% SDS-PAGE

All lanes:

Western blot - Anti-53BP1 antibody (ab21083) at 1/500 dilution

Lane 1:

53BP1 shRNA non-transfected HeLa whole cell extracts at 50 µg

Lane 2:

53BP1 shRNA transfected HeLa whole cell extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 214 kDa

Observed band size: 350 kDa

false