Anti-6X His tag® antibody [AD1.1.10]

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human GDAP1L1.

In Western blot, Anti-His tag antibody - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-GDAP1 antibody [EPR29581-48] (<a href='/ko/products/primary-antibodies/gdap1-antibody-epr29581-48-ab323844'>ab323844</a>) at 1/1000 dilution

Lane 1:

Purified 293T cells transfected with a human GDAP1 expression vector containing a His-tag at 20 µg

Lane 2:

Purified 293T cells transfected with a human GDAP1L1 expression vector containing a His-tag at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Beta-synuclein.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Alpha-synuclein (N-term) antibody [EPR29522-6] (<a href='/ko/products/primary-antibodies/alpha-synuclein-n-term-antibody-epr29522-6-ab324977'>ab324977</a>) at 1/1000 dilution

Lane 1:

His-tagged human Alpha-synuclein recombinant protein at 10 ng

Lane 2:

His-tagged human Beta-synuclein recombinant protein at 10 ng

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 18 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human ACSL1 and ACSL6 by Western Blot.

In Western blot, Anti-His tag® antibody (ab15149) (1 : 1000) (75-150KDa).

All lanes:

Western blot - Anti-ACSL5 antibody [EPR30222-556] (<a href='/ko/products/primary-antibodies/acsl5-antibody-epr30222-556-ab327086'>ab327086</a>) at 1/1000 dilution

Lane 1:

Non-tagged human ACSL5 recombinant fragment at 10 ng

Lane 2:

His-tagged human ACSL1 recombinant protein at 10 ng

Lane 3:

His-tagged human ACSL6 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 70 kDa,75-150 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human SV2B or SV2A by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-SV2C antibody [EPR30548-547] (<a href='/ko/products/primary-antibodies/sv2c-antibody-epr30548-547-ab324233'>ab324233</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human SV2C expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human SV2B expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Lane 4:

293T cells transfected with a human SV2A expression vector containing a myc-His-tag®, whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 82 kDa,36 kDa,77-82 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human THRA by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) & Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/200000 and 1 : 1000 dilutions respectively.

All lanes:

Western blot - Anti-Thyroid Hormone Receptor beta antibody [EPR28919-182] (<a href='/ko/products/primary-antibodies/thyroid-hormone-receptor-beta-antibody-epr28919-182-ab324308'>ab324308</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human THRB expression vector containi a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human THRA expression vector containi a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 52 kDa,54 kDa,36 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [AD1.1.10](ab15149)(1 : 1000)(16KDa).

All lanes:

Western blot - Anti-RPL26+RPL26L antibody [EPR28600-70] (<a href='/ko/products/primary-antibodies/rpl26rpl26l-antibody-epr28600-70-ab325434'>ab325434</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human RPL26 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human RPL26L expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 17 kDa,36 kDa,16 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human ITPR2 and ITPR1.

In Western blot, Anti-His tag® antibody (ab15149) (1 : 1000)(30-75KDa);Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-ITPR3 antibody [EPR25276-304] (<a href='/ko/products/primary-antibodies/itpr3-antibody-epr25276-304-ab325503'>ab325503</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a human ITPR3 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human ITPR2 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human ITPR1 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 74 kDa,30-75 kDa,36 kDa

false

Exposure time: 10s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human CISD1 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) (1 : 1000) (60KDa).

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602).

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - Loading Control (ab15149).

All lanes:

Western blot - Anti-Prosurfactant Protein C antibody (<a href='/ko/products/primary-antibodies/prosurfactant-protein-c-antibody-ab90716'>ab90716</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T transfected with a human SFTPC expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a mouse SFTPC expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 21 kDa

false

Exposure time: 4s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human MTHFD1L by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-MTHFD1 antibody [EPR29810-508] (<a href='/ko/products/primary-antibodies/mthfd1-antibody-epr29810-508-ab324379'>ab324379</a>) at 1/1000 dilution

Lane 1:

No-tagged human MTHFD1 fragment at 10 ng

Lane 2:

His-tagged human MTHFD1L fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,30 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human VPS4B by western blot.

In Western blot, Anti-His tag antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-VPS4a antibody [EPR30587-510] (<a href='/ko/products/primary-antibodies/vps4a-antibody-epr30587-510-ab325430'>ab325430</a>) at 1/1000 dilution

Lane 1:

His-tagged human VPS4A recombinant protein at 10 ng

Lane 2:

His-tagged human VPS4B recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa,34 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human SNX1 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) (1 : 1000) (60KDa).

All lanes:

Western blot - Anti-SNX2 antibody [EPR30535-531] (<a href='/ko/products/primary-antibodies/snx2-antibody-epr30535-531-ab326422'>ab326422</a>) at 1/1000 dilution

Lane 1:

His-tagged human SNX2 fragment at 10 ng

Lane 2:

His-tagged human SNX1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa

false

Exposure time: 8s

• Dot

Supplier Data

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of N-terminal LPHN2 using ab324484 at 1 : 1000 (0.495 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human LPHN2 recombinant fragment
Lane2 : His-tagged human LPHN1 recombinant fragment
Lane3 : His-tagged human LPHN3 recombinant fragment

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human LPHN1 or LPHN3.

#In Dot Blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-N-terminal LPHN2 antibody [EPR29557-9] (<a href='/ko/products/primary-antibodies/n-terminal-lphn2-antibody-epr29557-9-ab324484'>ab324484</a>) at 1/1000 dilution

Lane 1:

His-tagged human LPHN2 recombinant fragment

Lane 2:

His-tagged human LPHN1 recombinant fragment

Lane 3:

His-tagged human LPHN3 recombinant fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of JAK1 using ab324683 at 1 : 1000 (0.506 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human JAK1 recombinant fragment
Lane2 : His-tagged human JAK2 recombinant fragment
Lane3 : His-tagged human JAK3 recombinant fragment
Lane4 : His-tagged human TYK2 recombinant fragment

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human JAK2, JAK3 or TYK2 by dot blot.

#In Dot Blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-JAK1 antibody [EPR24649-58] (<a href='/ko/products/primary-antibodies/jak1-antibody-epr24649-58-ab324683'>ab324683</a>) at 1/1000 dilution

Lane 1:

His-tagged human JAK1 recombinant fragment

Lane 2:

His-tagged human JAK2 recombinant fragment

Lane 3:

His-tagged human JAK3 recombinant fragment

Lane 4:

His-tagged human TYK2 recombinant fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• Dot

Lab

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of Talin 1 using ab327421 at 1 : 1000 (0.479 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : Untagged human Talin 1 fragment 10 ng

Lane 2 : His-tagged human Talin 2 fragment 10 ng

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Talin 2.

All lanes:

Dot Blot - Anti-Talin 1 antibody [EPR29641-30] (<a href='/ko/products/primary-antibodies/talin-1-antibody-epr29641-30-ab327421'>ab327421</a>) at 1/1000 dilution

Lane 1:

Untagged human Talin 1 fragment at 10 ng

Lane 2:

His-tagged human Talin 2 fragment at 10 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of FURIN using ab324440 at 1 : 1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : No-tagged human PCSK3 recombinant protein
Lane2 : His-tagged human PCSK1 recombinant protein
Lane3 : His-tagged human PCSK4 recombinant protein
Lane4 : His-tagged human PCSK5 recombinant protein
Lane5 : His-tagged human PCSK6 recombinant protein

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PCSK1, PCSK4, PCSK5, and PCSK6.

In Dot blot, anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-FURIN antibody [RM2083] (<a href='/ko/products/primary-antibodies/furin-antibody-rm2083-ab324440'>ab324440</a>) at 1/1000 dilution

Lane 1:

No-tagged human PCSK3 recombinant protein

Lane 2:

His-tagged human PCSK1 recombinant protein

Lane 3:

His-tagged human PCSK4 recombinant protein

Lane 4:

His-tagged human PCSK5 recombinant protein

Lane 5:

His-tagged human PCSK6 recombinant protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with rat NPR2 by western blot.

In Western blot, Anti-His tag antibody (ab15149) (1 : 1000)(60KDa).

All lanes:

Western blot - Anti-NPR-A/Atrial natriuretic peptide receptor 1 antibody [EPR30453-586] (<a href='/ko/products/primary-antibodies/npr-a-atrial-natriuretic-peptide-receptor-1-antibody-epr30453-586-ab325540'>ab325540</a>) at 1/1000 dilution

Lane 1:

No-tagged rat NPR1 recombinant protein at 10 ng

Lane 2:

His-tagged rat NPR2 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 60 kDa

false

Exposure time: 3s

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with an empty expression vector containing GFP-6xHis-tag^® labelling 6X His tag with ab15149 at 1/250 dilution (4.456 μg/ml), followed by a secondary antibody ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) antibody at 1/500 dilution (4μg/ml) (Red).

Confocal image showing positive staining in HEK-293T cells transfected with an empty expression vector containing GFP-6xHis-tag^®. Nuclear DNA was labeled with DAPI (shown in blue).

• Dot

Lab

Dot Blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Dot blot analysis of TEAD4 using ab326726 at 1 : 1000 (0.506 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : His-tagged mouse TEAD4 recombinant protein

Lane 2 : His-tagged mouse TEAD1 recombinant protein

Lane 3 : His-tagged mouse TEAD3 recombinant protein

Lane 4 : His-tagged mouse TEAD2 recombinant protein

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse TEAD1, TEAD2 and TEAD3.

All lanes:

Dot Blot - Anti-TEAD4 antibody [EPR30556-16] (ab326726) at 1/1000 dilution

Lane 1:

His-tagged mouse TEAD4 recombinant protein

Lane 2:

His-tagged mouse TEAD1 recombinant protein

Lane 3:

His-tagged mouse TEAD3 recombinant protein

Lane 4:

His-tagged mouse TEAD2 recombinant protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

• I-ELISA

Lab

Indirect ELISA - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Indirect ELISA analysis of ab15149 at 5000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution.

Antigen : 6X His tag®.

Antigen concentration : 1000 ng/ml

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human UBQLN2 or UBQLN3 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

In Western blot, Anti-LIF antibody [EPR21713-25] - (ab244795) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Ubiquilin/UBQLN1 antibody [EPR29585-56] (<a href='/ko/products/primary-antibodies/ubiquilin-ubqln1-antibody-epr29585-56-ab325320'>ab325320</a>) at 1/1000 dilution

Lane 1:

His-tagged human UBQLN1 recombinant protein at 10 ng

Lane 2:

His-tagged human UBQLN2 recombinant protein at 10 ng

Lane 3:

LIF-tagged human UBQLN3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human CST4 and CST1.

In Western blot, Anti-His tag® antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (<a href='/ko/products/primary-antibodies/cystatin-sa-cst2-antibody-epr25934-14-ab325126'>ab325126</a>) at 1/1000 dilution

Lane 1:

Untagged human Cystatin SA/CST2 recombinant protein at 10 ng

Lane 2:

His-tagged human CST4 recombinant protein at 10 ng

Lane 3:

His-tagged human CST1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 14 kDa,15-16 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse ZIC1, ZIC2, ZIC4 and ZIC5.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody AD1.1.10 staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ZIC3 antibody [EPR30592-522] (<a href='/ko/products/primary-antibodies/zic3-antibody-epr30592-522-ab325172'>ab325172</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a mouse ZIC3 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse ZIC1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse ZIC2 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse ZIC4 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a mouse ZIC5 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,36 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed mouse Angiopoietin 2, Angiopoietin 3 and Angiopoietin 4 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Angiogenin antibody [RM2092] (<a href='/ko/products/primary-antibodies/angiogenin-antibody-rm2092-ab325241'>ab325241</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Angiogenin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a mouse Angiopoietin 2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a mouse Angiopoietin 3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a mouse Angiopoietin 4 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 16 kDa,36 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

In Western blot, Anti-6X His tag® antibody [AD1.1.10] total protein control and secondary antibody used was Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

ab32560 does not react with other 14-3-3 protein families.

All lanes:

Western blot - Anti-14-3-3 alpha + beta antibody [Y62] (<a href='/ko/products/primary-antibodies/14-3-3-alpha-beta-antibody-y62-ab32560'>ab32560</a>) at 1/1000 dilution

Lane 1:

Human 14-3-3 beta (YWHAB) Recombinant Protein with a his tag

Lane 2:

Human 14-3 3 gamma (YWHAG) Recombinant Protein with a his tag

Lane 3:

Human 14-3-3 epsilon (YWHAE) Recombinant Protein with a his tag

Lane 4:

Human 14-3-3 zeta (YWHAZ) Recombinant Protein with a his tag

Lane 5:

Human 14 3 3 eta (YWHAH) Recombinant Protein with a his tag

Lane 6:

Human 14-3-3 theta (YWHAQ) Recombinant Protein with a his tag

Lane 7:

Human 14-3-3 sigma (SFN) Recombinant Protein with a his tag

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30 kDa

false

Exposure time: 42s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody cross-reacts with overexpressed mouse PRSS2 and PRSS3 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Trypsin antibody [EPR29805-7] (<a href='/ko/products/primary-antibodies/trypsin-antibody-epr29805-7-ab325233'>ab325233</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with mouse PRSS1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with mouse PRSS2 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T transfected with mouse PRSS3 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26 kDa,28 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human CAPN1, CAPN3, CAPN8, CAPN9 or CAPN11 by western blot.

In Western blot, Anti-His tag antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Calpain 2 antibody [EPR29833-94] (<a href='/ko/products/primary-antibodies/calpain-2-antibody-epr29833-94-ab325140'>ab325140</a>) at 1/1000 dilution

Lane 1:

His-tagged human CAPN2 fragment at 10 ng

Lane 2:

His-tagged human CAPN1 fragment at 10 ng

Lane 3:

His-tagged human CAPN3 fragment at 100 ng

Lane 4:

His-tagged human CAPN8 fragment at 10 ng

Lane 5:

His-tagged human CAPN9 fragment at 10 ng

Lane 6:

His-tagged human CAPN11 fragment at 10 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 34 kDa

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

In Western blot, Anti-Anti-6X His tag® antibody [AD1.1.10] loading control staining at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human light chain Factor XII.

All lanes:

Western blot - Anti-Factor XII antibody [EPR22292-127] (<a href='/ko/products/primary-antibodies/factor-xii-antibody-epr22292-127-ab242123'>ab242123</a>) at 1/1000 dilution

Lane 1:

His-tagged heavy chain of human Factor XII recombinant protein at 10 ng

Lane 2:

Lif/His-tagged light chain of human Factor XII recombinant protein at 10 ng

Lane 3:

No-tagged human Factor XII recombinant protein without His tag at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 65 kDa,50-70 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag^® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ESM1 antibody [RM1338] (<a href='/ko/products/primary-antibodies/esm1-antibody-rm1338-ab323845'>ab323845</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a human ESM1 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse TAP2.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-His tag antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-TAP1 antibody [EPR30551-512] (<a href='/ko/products/primary-antibodies/tap1-antibody-epr30551-512-ab324718'>ab324718</a>) at 1/1000 dilution

Lane 1:

Untagged mouse TAP1 fragment at 10 ng

Lane 2:

His-tagged mouse TAP2 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa,34 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human PYCR1 or PYCR3 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-PYCR2 antibody [EPR28825-54] (<a href='/ko/products/primary-antibodies/pycr2-antibody-epr28825-54-ab324352'>ab324352</a>) at 1/1000 dilution

Lane 1:

His-tagged human PYCR1 recombinant protein at 10 ng

Lane 2:

His-tagged human PYCR2 recombinant protein at 10 ng

Lane 3:

His-tagged human PYCR3 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa,33 kDa,29 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : QD20250827IMG37.

This antibody does not cross-react with recombinant human FCN2 or FCN1 by western blot.

In Western blot, Anti-6X His tag® antibody [AD1.1.10]- (ab15149) staining at 1/1000 dilution.

Exposure time : Lane 1 : 3 seconds; Lanes 2-4 : 10 seconds.

All lanes:

Western blot - Anti-Ficolin-3 antibody [EPR30745-69] (<a href='/ko/products/primary-antibodies/ficolin-3-antibody-epr30745-69-ab324673'>ab324673</a>) at 1/1000 dilution

Lane 1:

Human FCN3 recombinant protein (no-tag) at 10 ng

Lane 2:

His-tagged human FCN2 recombinant protein at 10 ng

Lane 3:

His-tagged human FCN1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa,26 kDa

false

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] - (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-GPA33 antibody [EPR30348-518] (<a href='/ko/products/primary-antibodies/gpa33-antibody-epr30348-518-ab324562'>ab324562</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a mouse GPA33 expression vector containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 43 kDa,36 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-6X His tag® antibody [AD1.1.10] (ab15149) at 1/5000 dilution

Lane 1:

His-tagged mouse Cathepsin D recombinant protein (aa65-310; 27kDa)

Lane 2:

His-tagged mouse CDKN2A/p16INK4a recombinant protein (aa1-168;18kDa)

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 27 kDa,18 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was used at a 1/200000 dilution. Anti-6X His tag® antibody [AD1.1.10](ab15149) used for total protein control.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Pneumolysin antibody [PLY-4] (<a href='/ko/products/primary-antibodies/pneumolysin-antibody-ply-4-ab71810'>ab71810</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 40 µg

Lane 2:

293T transfected with Streptococcus pneumoniae serotype 2 (strain D39 / NCTC 7466) expression vector containing a myc-His-tag® whole cell lysate at 40 µg

Lane 3:

293T transfected with Streptococcus pyogenes serotype M3 (strain SSI-1) expression vector containing a myc-His-tag® whole cell lysate at 40 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 53 kDa

true

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Rift Valley Fever Virus Nucleoprotein antibody [AA11] (<a href='/ko/products/primary-antibodies/rift-valley-fever-virus-nucleoprotein-antibody-aa11-ab325011'>ab325011</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with Rift valley fever virus (RVFV) Nucleoprotein (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 1 µg

Lane 2:

293T cells transfected with Rift valley fever virus (RVFV) Envelopment polyprotein (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with Rift valley fever virus (RVFV) RNA-directed RNA polymerase L (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with Rift valley fever virus (RVFV) Non-structural protein S (strain ZH-548 M12) containing a myc-his-tag whole cell lysate at 5 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) (ZB-2305) at 1/10000 dilution

Observed band size: 28-243 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Nipah Virus Glycoprotein F antibody [CG11] (<a href='/ko/products/primary-antibodies/nipah-virus-glycoprotein-f-antibody-cg11-ab325231'>ab325231</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with Nipah virus Fusion glycoprotein F0 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with Hendra virus (isolate Horse/Autralia/Hendra/1994) Fusion glycoprotein F0 expression vector containing a myc-His-tag® whole cell lysate at 2 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 60 kDa,36 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1 : 1000 dilution.

All lanes:

Western blot - Anti-Lassa Fever virus GP1 antibody [HA6] (<a href='/ko/products/primary-antibodies/lassa-fever-virus-gp1-antibody-ha6-ab325160'>ab325160</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with Lassa virus (strain GA391) Glycoprotein G1(GP1) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with Lassa virus (strain GA391) Glycoprotein G2(GP2) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T transfected with Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) Glycoprotein G1(GP1) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 5:

293T transfected with Lassa virus (strain Mouse/Sierra Leone/Josiah/1976) Glycoprotein G2(GP2) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 25 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human ETV1 and ETV5.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 37002241).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Pea3 antibody [EPR30314-30] (<a href='/ko/products/primary-antibodies/pea3-antibody-epr30314-30-ab323843'>ab323843</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human ETV4 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human ETV1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human ETV5 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 54-61 kDa,36 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-His tag® antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Dengue NS3 antibody [EPR29361-53] (<a href='/ko/products/primary-antibodies/dengue-ns3-antibody-epr29361-53-ab324565'>ab324565</a>) at 1/5000 dilution

Lane 1:

293T (human epithelial cell line from embryonic kidney) cells transfected with an empty vector containing a Myc-His tag, whole cell lysate at 20 µg

Lane 2:

293T transfected with a Virus Dengue type 2 NS3 expression vector containing a Myc-His tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a Virus Dengue type 4 NS3 expression vector containing a Myc-His tag, whole cell lysate at 20 µg

Lane 4:

293T transfected with a Virus Dengue type 3 NS3 expression vector containing a Myc-His tag, whole cell lysate at 20 µg

Lane 5:

293T transfected with a Virus Dengue type 1 NS3 expression vector containing a Myc-His tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 6s

• WB

Lab

Western blot - Anti-6X His tag® antibody [AD1.1.10] (AB15149)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);Anti-6X His tag® antibody [AD1.1.10] (ab15149) (27-150KDa).

All lanes:

Western blot - Anti-CD66b antibody [RM1323] (<a href='/ko/products/primary-antibodies/cd66b-antibody-rm1323-ab325777'>ab325777</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a His-tag, whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human CEACAM1/CD66a expression vector containing a His-tag, whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human CEACAM3/CD66d expression vector containing a His-tag, whole cell lysate at 5 µg

Lane 4:

293T cells transfected with a human CEACAM5/CD66e expression vector containing a His-tag, whole cell lysate at 5 µg

Lane 5:

293T cells transfected with a human CEACAM6/CD66c expression vector containing a His-tag, whole cell lysate at 5 µg

Lane 6:

293T cells transfected with a human CEACAM8/CD66b expression vector containing a His-tag, whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 95 kDa,36 kDa,27-150 kDa

false

Exposure time: 15s