Anti-6X His tag® antibody [EPR20547] - ChIP Grade

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling 6X His tag® with ab213204 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Negative control : No staining on human liver.

Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Immunohistochemical analysis of agarose-embedded HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged Staphylococcus aureus cas9 (J7RUA5; aa1-1053; 125kDa) construct labeling 6X His tag® with ab213204 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Left image : Positive staining on HEK-293T transfected with a His-tagged Staphylococcus aureus cas9 (J7RUA5; aa1-1053; 125kDa) construct. Right image : No staining on HEK-293T transfected with an empty expression vector.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-Myc-His vector labeling 6X His tag® with ab213204 (right panel) at 1/5000 dilution compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) at 1/2000 dilution was used as the secondary antibody.

Gate is set between transfected and untransfected HEK-293T cells.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with GFP-Myc-His vector expression construct labeling 6X His tag® with ab213204 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (white). Confocal image showing positive staining on HEK-293T cells transfected with GFP-Myc-His vector expression construct.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• ChIP

Supplier Data

ChIP - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Chromatin was prepared from MCF7 (human breast adenocarcinoma cell line) cells transfected with 6X His-tagged GATA3 according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25μg of chromatin, 2μg of ab213204 (blue), and 20μl of A/G sepharose beads slurry (10μl of sepharose A beads + 10μl of sepharose G beads). 2μg of rabbit normal IgG was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

ChIP was performed according to the literature (PMID : 22951069).

• IP

Supplier Data

Immunoprecipitation - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

His-tagged Staphylococcus aureus cas9 was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a His-tagged Staphylococcus aureus cas9 (J7RUA5; aa1-1053; 125 kDa) construct, whole cell lysate with ab213204 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213204 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : HEK-293T transfected with His-tagged Staphylococcus aureus cas9 construct, whole cell lysate 10 μg (Input).

Lane 2 : ab213204 IP in HEK-293T transfected with His-tagged Staphylococcus aureus cas9 construct, whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213204 in HEK-293T transfected with His-tagged Staphylococcus aureus cas9 construct, whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)

Observed band size: 125 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling 6X His tag® with ab213204 at 1/16000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Negative control : No staining on rat stomach.

Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-NCBP2AS2/KRASIM antibody [EPR28845-5] (<a href='/ko/products/primary-antibodies/ncbp2as2-antibody-epr28845-5-ab323482'>ab323482</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human NCBP2AS2 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Observed band size: 16 kDa,36 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human VPS4B by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(48KDa).

All lanes:

Western blot - Anti-VPS4a antibody [EPR30587-24] (<a href='/ko/products/primary-antibodies/vps4a-antibody-epr30587-24-ab327386'>ab327386</a>) at 1/1000 dilution

Lane 1:

His-tagged human VPS4A recombinant protein at 10 ng

Lane 2:

His-tagged human VPS4B recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 48 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-VE Cadherin (phospho Y685) antibody [EPR27437-539] (<a href='/ko/products/primary-antibodies/ve-cadherin-phospho-y685-antibody-epr27437-539-ab325018'>ab325018</a>) at 1/5000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human wild-type VE Cadherin expression vector containing a his-tag were treated with 100uM Pervanadate for 10 mins, whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human VE Cadherin (Y685A mutant) expression vector containing a his-tag were treated with 100uM Pervanadate for 10 mins, whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with wild-type human POLE.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa); Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) (1 : 5000).

All lanes:

Western blot - Anti-POLE (mutated V411L) antibody [EPR30786-591] (<a href='/ko/products/primary-antibodies/pole-mutated-v411l-antibody-epr30786-591-ab328037'>ab328037</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human POLE(V411L) expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human wild-type POLE expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 261 kDa,124 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human TRAK2.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(130KDa).

All lanes:

Western blot - Anti-TRAK1 antibody [EPR30574-567] (<a href='/ko/products/primary-antibodies/trak1-antibody-epr30574-567-ab327353'>ab327353</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human TRAK1 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human TRAK2 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human TRPM6

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-TRPM7 antibody [EPR27067-52] (<a href='/ko/products/primary-antibodies/trpm7-antibody-epr27067-52-ab323270'>ab323270</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human TRPM7 expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human TRPM6 expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 250 kDa,124 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (83KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Hormone sensitive lipase/HSL (phospho S660) antibody [EPR30648-593] (<a href='/ko/products/primary-antibodies/hormone-sensitive-lipase-hsl-phospho-s660-antibody-epr30648-593-ab326331'>ab326331</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate (untreated membrane) at 5 µg

Lane 2:

293T cells transfected with a human wild-type HSL expression vector containing a myc-His-tag® whole cell lysate (untreated membrane) at 5 µg

Lane 3:

293T cells transfected with a human wild-type HSL expression vector containing a myc-His-tag®, treated with 100nM Calyculin A for 30 minutes whole cell lysate (untreated membrane) at 5 µg

Lanes 4 and 8:

293T cells transfected with a human HSL (mutation S650A) expression vector containing a myc-His-tag®, treated with 100nM Calyculin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 5 µg

Lane 5:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate (alkaline phosphatase treated membrane) at 5 µg

Lane 6:

293T cells transfected with a human wild-type HSL expression vector containing a myc-His-tag® whole cell lysate (alkaline phosphatase treated membrane) at 5 µg

Lane 7:

293T cells transfected with a human wild-type HSL expression vector containing a myc-His-tag®, treated with 100nM Calyculin A for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 83 kDa,36 kDa

false

Exposure time: 6s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human DLL3 or DLL4 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(85, 30KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-DLL1 antibody [EPR29655-26] (<a href='/ko/products/primary-antibodies/dll1-antibody-epr29655-26-ab327088'>ab327088</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human DLL1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human DLL3 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human DLL4 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 85 kDa,30 kDa,36 kDa

false

Exposure time: 8s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The antibody shows weak reactivity with HEK293T cell lines overexpressing human Ret (Y1062A mutant)

In Western blot, Anti-Ret antibody (ab134100) (1 : 1000) (150KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(150KDa); Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) 1 : 100000 (15Kda).

All lanes:

Western blot - Anti-Ret (phospho Y1062) antibody [EPR30639-507] (<a href='/ko/products/primary-antibodies/ret-phospho-y1062-antibody-epr30639-507-ab326572'>ab326572</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag treated with 100uM pervanadate for 10 min whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human wild-type Ret expression vector containing a his-tag were treated with 100uM Pervanadate for 10 mins, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human Ret(Y1062A mutant) expression vector containing a his-tag were treated with 100uM Pervanadate for 10 mins, whole cell lysate at 2 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 150 kDa,15 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human BRG1.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-SMARCA2 / BRM antibody [EPR28611-92] (<a href='/ko/products/primary-antibodies/smarca2-brm-antibody-epr28611-92-ab323179'>ab323179</a>) at 1/1000 dilution

Lane 1:

His-tagged human SMARCA2/BRM fragment at 10 ng

Lane 2:

His-tagged human BRG1 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa,36 kDa,20-36 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The 140 kDa band detected in the blot represents the endogenous form of SA2 (isoform-1).

This antibody does not cross-react with recombinant human SA2 (isoform-2) by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) & Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)staining at 1/200000 and 1/5000 dilutions respectively.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-SA2 (isoform-1) antibody [EPR30102-55] (<a href='/ko/products/primary-antibodies/sa2-isoform-1-antibody-epr30102-55-ab324732'>ab324732</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 2:

293T transfected with a human SA2 (isoform-1) expression vector containing a myc-His-tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a human SA2 (isoform-2) expression vector containing a myc-His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 17-30 kDa,140 kDa,36 kDa,14-30 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody specifically recognizes the HDGFL2 cryptic peptide.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (140KDa).

All lanes:

Western blot - Anti-HDGFL2 cryptic peptide antibody [EPR31252-542] (ab328148) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human HDGFL2 cryptic peptide expression vector containing a his-tag, whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human wild-type HDGFL2 expression vector containing a his-tag, whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140 kDa,36 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human SLC34A1 or SLC34A3 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (75-100KDa).

All lanes:

Western blot - Anti-SLC34A2 antibody [EPR30528-645] (<a href='/ko/products/primary-antibodies/slc34a2-antibody-epr30528-645-ab325747'>ab325747</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 2:

293T cells transfected with human SLC34A2 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 3:

293T cells transfected with human SLC34A1 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 4:

293T cells transfected with human SLC34A3 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75-150 kDa,36 kDa,75-100 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(24-30KDa).

All lanes:

Western blot - Anti-IL-31 antibody [EPR31053-655] (ab328153) at 1/1000 dilution

Lane 1:

Untreated 293T (human embryonic kidney epithelial cell) cells transfected with a human IL-31expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human IL-31 expression vector containing a his-tag, whole cell lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 24-30 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Loading Control (ab176842) (1 : 100000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(150-250kDa).

All lanes:

Western blot - Anti-SOS1 (phospho Y1196) antibody [TR16-7-3] (<a href='/ko/products/primary-antibodies/sos1-phospho-y1196-antibody-tr16-7-3-ab325592'>ab325592</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with a human wild-type SOS1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T transfected with a human SOS1 (Y1196A mutation) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 152 kDa,36 kDa,150-250 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human EMP1 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (20-50KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-EMP2 antibody [EPR30305-511] (<a href='/ko/products/primary-antibodies/emp2-antibody-epr30305-511-ab326423'>ab326423</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human EMP1 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human EMP2 expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20-50 kDa,36 kDa

false

Exposure time: 37s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

293T cells express endogenously BCL9. This antibody does not cross-react with human BCL9L by WB.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(200KDa).

All lanes:

Western blot - Anti-BCL9 antibody [EPR29651-74] (<a href='/ko/products/primary-antibodies/bcl9-antibody-epr29651-74-ab325937'>ab325937</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a his-tag, fresh whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human BCL9 expression vector containing a his-tag, fresh whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human BCL9L expression vector containing a his-tag, fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 200 kDa,124 kDa

false

Exposure time: 48s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human ZMIZ2 by western blot.

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible. ZMIZ1 can undergo degradation easily.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(150KDa).

All lanes:

Western blot - Anti-Zinc finger MIZ domain-containing protein 1 antibody [EPR29807-525] (<a href='/ko/products/primary-antibodies/zinc-finger-miz-domain-containing-protein-1-antibody-epr29807-525-ab326070'>ab326070</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human ZMIZ2 expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human ZMIZ1 expression vector containing a myc-His-tag® fresh whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 120 kDa,130 kDa,150 kDa,36 kDa

false

Exposure time: 10s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (50 KDa).

All lanes:

Western blot - Anti-Acetyl-CBP (Lys1535)/p300 (Lys1499) antibody [EPR28740-568] (<a href='/ko/products/primary-antibodies/acetyl-cbp-lys1535-p300-lys1499-antibody-epr28740-568-ab325530'>ab325530</a>) at 1/1000 dilution

Lane 1:

Purified 293T(human embryonic kidney epithelial cell) transfected with a human wild-type p300 HAT domain expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

Purified 293T transfected with a human p300 HAT domain(K1499A mutation) expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa

false

Exposure time: 70s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (50kDa);
Anti-Vinculin antibody [EPR8185] (ab129002) (1 : 10000) (124KDa);
Anti-PDGFR alpha antibody [EPR22059-270] - (ab203491) (1 : 1000) (160KDa).

All lanes:

Western blot - Anti-PDGFR alpha (phospho Y754) antibody [EPR31185-28] (<a href='/ko/products/primary-antibodies/pdgfr-alpha-phospho-y754-antibody-epr31185-28-ab327840'>ab327840</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 2:

293T cells transfected with a human PDGFR alpha (WT) expression vector containing a myc-His-tag® whole cell lysate at 5 µg

Lane 3:

293T cells transfected with a human PDGFR alpha (WT) expression vector containing a myc-His-tag®, and then treated with 1mM pervanadate for 30min whole cell lysate at 5 µg

Lane 4:

293T cells transfected with a human PDGFR alpha (Y754A mutant) expression vector containing a myc-His-tag®, and then treated with 1mM pervanadate for 30min whole cell lysate at 5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 160 kDa,124 kDa

false

Exposure time: 3s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human IFNL2 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (21KDa);.

All lanes:

Western blot - Anti-IFNL3 antibody [EPR30983-3] (<a href='/ko/products/primary-antibodies/ifnl3-antibody-epr30983-3-ab327709'>ab327709</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human IFNL3 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human IFNL2 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 21 kDa,36 kDa

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human CCL2, CCL11or CCL7.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);
Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(15KDa).

All lanes:

Western blot - Anti-MCP2 antibody [EPR29033-501] (<a href='/ko/products/primary-antibodies/mcp2-antibody-epr29033-501-ab327462'>ab327462</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 4 µg

Lane 2:

293T cells transfected with a human CCL8 expression vector containing a his-tag, whole cell lysate at 4 µg

Lane 3:

293T cells transfected with a human CCL2 expression vector containing a his-tag, whole cell lysate at 4 µg

Lane 4:

293T cells transfected with a human CCL11 expression vector containing a his-tag, whole cell lysate at 4 µg

Lane 5:

293T cells transfected with a human CCL7 expression vector containing a his-tag, whole cell lysate at 4 µg

Lane 6:

293T cells transfected with a human CCL13 expression vector containing a his-tag, whole cell lysate at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa,36 kDa

false

Exposure time: 70s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human BACH2 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(48KDa).

All lanes:

Western blot - Anti-BACH1.3 antibody [EPR29608-726] (<a href='/ko/products/primary-antibodies/bach13-antibody-epr29608-726-ab324640'>ab324640</a>) at 1/1000 dilution

Lane 1:

His-tagged human BACH1.3 fragment at 10 ng

Lane 2:

His-tagged human BACH2 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 48 kDa

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human COL1A1, COL2A1, COL3A1 or COL5A2 by Western Blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-COL1A2 antibody [EPR29717-534] (<a href='/ko/products/primary-antibodies/col1a2-antibody-epr29717-534-ab323563'>ab323563</a>) at 1/1000 dilution

Lane 1:

His-tagged human COL1A2 recombinant protein at 10 ng

Lane 2:

His-tagged human COL2A1 recombinant protein at 10 ng

Lane 3:

His-tagged human COL1A1 recombinant protein at 10 ng

Lane 4:

His-tagged human COL3A1 recombinant protein at 10 ng

Lane 5:

His-tagged human COL5A2 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 28 kDa

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands at 37 kDa and 20 kDa are unknown.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(76KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-MAP3K20 (phospho S7) antibody [EPR28256-30] (<a href='/ko/products/primary-antibodies/map3k20-phospho-s7-antibody-epr28256-30-ab324636'>ab324636</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human wild-type MAP3K20 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human MAP3K20 (S7A mutant) expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 76 kDa,36 kDa

false

Exposure time: 26s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human MAPK8IP1, MAPK8IP2 or MAPK8IP4.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(240KDa).

All lanes:

Western blot - Anti-JIP3 antibody [EPR30425-79] (<a href='/ko/products/primary-antibodies/jip3-antibody-epr30425-79-ab326560'>ab326560</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a human MAPK8IP3 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human MAPK8IP1 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human MAPK8IP2 expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human MAPK8IP4 expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 240 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does recognize all the CT45A family members 1-10.

The bands beneath the target band (25 kDa) are likely to be degraded target fragments.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 23758873).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-CT45 antibody [EPR29691-71] (<a href='/ko/products/primary-antibodies/ct45-antibody-epr29691-71-ab323553'>ab323553</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human CT45A1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human CT45A2/8/9 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human CT45A3/4 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 5:

293T cells transfected with a human CT45A5/6/7 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 6:

293T cells transfected with a human CT45A10 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 25 kDa,36 kDa,15-25 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking buffer and concentration : 1% BSA/TBST
Diluting buffer and concentration : 5% NFDM/TBST

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa);Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(125KDa).

All lanes:

Western blot - Anti-ATP citrate lyase (phospho S455) antibody [EPR31107-40] (<a href='/ko/products/primary-antibodies/atp-citrate-lyase-phospho-s455-antibody-epr31107-40-ab327419'>ab327419</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a human wild-type ACLY expression vector containing a his-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human ACLY(S455A mutant) expression vector containing a his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 125 kDa,36 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag antibody [EPR20547]-CHIP Grade (ab213204) (1 : 5000)(35-50KDa).

All lanes:

Western blot - Anti-Cyclin E1(Phospho T77) antibody [EPR28889-10] (ab327426) at 1/5000 dilution

Lane 1:

293T cells transfected with a human wild-type CCNE1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human CCNE1 (T77A) expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/200000 dilution

Observed band size: 35-50 kDa,36 kDa

false

Exposure time: 15s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human CPLX2 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (17KDa).

All lanes:

Western blot - Anti-CPLX1 antibody [EPR31022-42] (<a href='/ko/products/primary-antibodies/cplx1-antibody-epr31022-42-ab327002'>ab327002</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a human CPLX1 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Lane 3:

293T cells transfected with a human CPLX2 expression vector containing a myc-His-tag® whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 17 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human MURF3 and MURF2.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(37-100KDa).

All lanes:

Western blot - Anti-MURF1 antibody [EPR29784-48] (<a href='/ko/products/primary-antibodies/murf1-antibody-epr29784-48-ab327341'>ab327341</a>) at 1/1000 dilution

Lane 1:

Untagged human MURF1 fragment at 10 ng

Lane 2:

His-tagged human MURF3 fragment at 10 ng

Lane 3:

His-tagged human MURF2 fragment at 10 ng

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 16 kDa,37-100 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with recombinant human SORCS3 by western blot.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa); Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (130KDa).

All lanes:

Western blot - Anti-SorCS1 antibody [EPR30536-522] (<a href='/ko/products/primary-antibodies/sorcs1-antibody-epr30536-522-ab325598'>ab325598</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

Lane 2:

293T transfected with a human SORCS1 expression vector containing a His-tag, whole cell lysate at 20 µg

Lane 3:

293T transfected with a human SORCS3 expression vector containing a His-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 130 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-6X His tag® antibody [EPR20547] - ChIP Grade (AB213204)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human CTSK, CTSS or CTSV.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000)(30-75KDa).

All lanes:

Western blot - Anti-Cathepsin L/MEP antibody [EPR30887-518] (<a href='/ko/products/primary-antibodies/cathepsin-l-mep-antibody-epr30887-518-ab326362'>ab326362</a>) at 1/1000 dilution

Lane 1:

No tagged human CTSL recombinant fragment at 10 ng

Lane 2:

His-tagged human CTSK recombinant fragment at 10 ng

Lane 3:

His-tagged human CTSS recombinant fragment at 10 ng

Lane 4:

His-tagged human CTSV recombinant fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa,30-75 kDa

false

Exposure time: 48s