AB300741

Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free)

RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(0 출판물)

Rabbit Recombinant Monoclonal 7-Methylguanosine antibody. Carrier free. Suitable for Dot, FRET and reacts with Modified Nucleic Acid samples.

대체 명칭 보기

m7G

5 이미지

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : HeLa total RNA Lane 2 : HeLa mRNA Exposure time : 137s /17s

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : m7G(cap)-oligo Lane 2 : m2A-oligo Lane 3 : m1G-oligo Lane 4 : m2, 2G-oligo Lane 5 : G-oligo Lane 6 : m2G-oligo Lane 7 : m1A-oligo Lane 8 : m6, 6A-oligo Exposure times : Lane 1 : 6 seconds; Lanes 2-8 : 180 seconds

Fluorescence Resonance Energy Transfer - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

FRET

Supplier Data

Fluorescence Resonance Energy Transfer - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. A FRET-based assay was performed using 25 nm of each biotinylated oligo and ab300740 at a concentration range of 0.001-4.000 ug/ml.

Supplier Data

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

This data was developed using ab300740, the same antibody clone in a different buffer formulation. Concentration of ab300740 : 1/500 dilution (1 μg/ml) Secondary ab : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051), 1/20000 dilution Blocking/diluting buffer and concentration : AdvanBlock Lane 1 : m7G(cap)-oligo Lane 2 : m2A-oligo Lane 3 : m1G-oligo Lane 4 : m2, 2G-oligo Lane 5 : G-oligo Lane 6 : m2G-oligo Lane 7 : m1A-oligo Lane 8 : m6, 6A-oligo Exposure time : lane 1, 6 sec.; lanes 2-8, 180 sec.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20846-92

Isotype

IgG

Carrier free

Yes

Applications

Dot, FRET

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FRET" : {"fullname" : "Fluorescence Resonance Energy Transfer", "shortname":"FRET"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Nucleic Acid": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "FRET-species-checked": "testedAndGuaranteed", "FRET-species-dilution-info": "", "FRET-species-notes": "<p></p>" } } }

style

left-column

제품 세부 정보

ab300741 is the carrier-free version of ab300740.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The target 7-Methylguanosine also known as 7-methylguanosine cap or 7-methylguanosine triphosphate (7-methylguanosine GTP) plays an important role in RNA processing. It is a modified nucleotide with a mass of approximately 362.3 Da. This cap structure is found at the 5' end of eukaryotic messenger RNA (mRNA). The 7-methylguanosine cap is expressed in the nucleus where it is added co-transcriptionally to nascent mRNA transcripts. This modification enhances mRNA stability and regulates its export to the cytoplasm.

Biological function summary

This cap modification promotes ribosomal binding facilitating efficient translation initiation. The 7-methylguanosine cap is part of the cap-binding complex which includes other proteins like eIF4E. This complex protects RNA from degradation and aids in splicing 5' end processing and polyadenylation. The methylation of the guanine base forms a structure that recruits proteins essential for mRNA maturation and function. Without this modification mRNA would display reduced translational efficiency and be more susceptible to exonucleolytic decay.

Pathways

7-methylguanosine participates in the RNA processing and translation initiation pathways. It interacts with the eukaryotic translation initiation factor 4E (eIF4E) a critical player in the regulation of mRNA cap-dependent translation. The binding of eIF4E to the 7-methylguanosine cap is essential for assembly of the eIF4F complex. This complex drives the mRNA to the ribosome facilitating protein synthesis. Additionally this cap structure influences mRNA export and poly(A) tail formation connecting to the broader mRNA metabolic pathway and impacting gene expression regulation.

Defects in the 7-methylguanosine cap can lead to improper mRNA processing and contribute to diseases like cancer and some congenital disorders. Dysregulation of cap-binding proteins such as eIF4E links to oncogenesis as these proteins can become overexpressed in cancers driving abnormal cell proliferation. Additionally cancer therapies often target these pathways to inhibit the translation of oncogenic mRNAs. Furthermore mutations or malfunctions in methylation enzymes can result in congenital developmental disorders as critical proteins necessary for normal growth and cellular function become deficient due to impaired mRNA translation.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

See full target information 7-Methylguanosine

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free)

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Fluorescence Resonance Energy Transfer - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

Dot Blot - Anti-7-Methylguanosine antibody [EPR20846-92] (BSA and Azide free) (AB300741)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise