Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1]

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (AB48508)

De-paraffinized and dehydrated sections were treated in a microwave oven at low power for 10 min in 0.01 M sodium citrate buffer (pH 6.0) to retrieve antigen, and inactivated endogenous peroxidase using 3% hydrogen peroxide in methanol for 10 min, and blocked with blocking solution for 1 hour at room temperature. Sections were then incubated with 1∶20 dilution of mouse monoclonal antibody to 8-Hydroxy-2'-deoxyguanosine (ab48508) overnight at 4°C. For detection, a biotinylated anti-mouse and HRP-labeled Streptavidin, and staining with DAB was used.

Immunohistochemical staining of nitrotyrosine (A) and 8-hydroxy-2′-deoxyguanosine (B) was performed on 5 rats randomly chosen from each group (ND : chow diet group, HFD : High fat diet, HFD-QH : High fat diet treated with Qing Huo Yi Hao" (QHYH)). An islet is encircled in each quandrant. Black arrows point to islets of Langerhans (Original magnification ×200). Percentage of immunohistochemial positive staining area in islet out of the area of corresponding islet (C, D) was presented. Values and bars represent means±SEM. * and ** denote P<0.05 and P<0.001 versus group ND.

Image from Wu L et al., PLoS ONE 8(4): e60262. Fig 6 doi:10.1371/journal.pone.0060262 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• IHC-P

AbReview23178****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (AB48508)

ab48508 staining 8-Hydroxy-2'-deoxyguanosine in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a TE buffer. Samples were incubated with primary antibody (1/50) for 30 mins at 20°C. A HRP-conjugated Goat anti-mouse IgG was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Rudolf Jung

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-8-Hydroxy-2'-deoxyguanosine antibody [N45.1] (AB48508)

ab48508, at a concentration of 5 μg/ml, staining 8-Hydroxy-2'-deoxyguanosine in epidermis from hairless mouse after 4 weeks of treatment by chronic UVB irradiation.