AB324855

Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal AAV2 antibody. Carrier free. Suitable for Dot, I-ELISA and reacts with Recombinant fragment - Adeno-associated virus 2, Recombinant fragment - Adeno-associated virus 3B samples.

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Indirect ELISA - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)

I-ELISA

Supplier Data

Indirect ELISA - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)

This data was developed using ab322961, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab322961 at 1000-0 ng/ml. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ Fragment Specific at 1 : 2000 dilution dilution.

Antigen : AAV1 empty capsid, AAV2 empty capsid, AAV3B empty capsid, AAV5 empty capsid, AAV6 empty capsid, AAV8 empty capsid, AAV9 empty capsid.

Antigen concentration : 1.0E+10 VP/ml

Supplier Data

Dot Blot - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)

This data was developed using ab322961, the same antibody clone in a different buffer formulation.

Dot blot analysis of AAV2 + AAV3B using ab322961 at 1 : 1000 (1.05 ug/ml) followed by a Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1 : 2000 dilution.

Lane1 : AAV1
Lane2 : AAV2
Lane3 : AAV3B
Lane4 : AAV5
Lane5 : AAV6
Lane6 : AAV8
Lane7 : AAV9

Exposure time : 59 seconds.

Blocking and diluting buffer and concentration : 2% BSA.

Anti-AAV2 antibody [EPR27877] (ab322961) (1 : 1000) recognizes AAV2/3B capsid.

All lanes:

Dot Blot - Anti-AAV2 + AAV3B antibody [EPR27877] (<a href='/ko/products/primary-antibodies/aav2-aav3b-antibody-epr27877-ab322961'>ab322961</a>) at 1/1000 dilution

Lane 1:

AAV1 at 10 ng

Lane 2:

AAV2 at 10 ng

Lane 3:

AAV3B at 10 ng

Lane 4:

AAV5 at 10 ng

Lane 5:

AAV6 at 10 ng

Lane 6:

AAB8 at 10 ng

Lane 7:

AAV9 at 10 ng

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/2000 dilution

false

Exposure time: 59s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

EPR27877

Isotype

IgG3

Carrier free

Yes

Reacts with

Adeno-associated virus 2, Adeno-associated virus 3B

Applications

I-ELISA, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody has the same CDR sequence as clone A20 as described in McCraw etal. Structure of adeno-associated virus-2 in complex with neutralizing monoclonal antibody A20. Virology 431: 40-49.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Adeno-associated virus 2": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Adeno-associated virus 3B": { "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Adeno-associated virus 2": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Adeno-associated virus 3B": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Transfected cell line - Adeno-associated virus 2": { "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }

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제품 세부 정보

ab324855 is the carrier-free version of ab322961.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Ion exchange chromatography

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information AAV2

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free

Indirect ELISA - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)

Dot Blot - Anti-AAV2 + AAV3B antibody [EPR27877] - BSA and Azide free (AB324855)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Assay Kits

Proteins & Peptides

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

Product promise