JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238989

Anti-ABCD1/ALD antibody [EPR15929] - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal ABCD1/ALD antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

대체 명칭 보기

ALD, ABCD1, ATP-binding cassette sub-family D member 1, Adrenoleukodystrophy protein, ALDP

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-ABCD1/ALD antibody [EPR15929] - BSA and Azide free (AB238989)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ABCD1/ALD antibody [EPR15929] - BSA and Azide free (AB238989)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ABCD1/ALD with ab197013 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab197013 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197013).

Flow Cytometry (Intracellular) - Anti-ABCD1/ALD antibody [EPR15929] - BSA and Azide free (AB238989)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ABCD1/ALD antibody [EPR15929] - BSA and Azide free (AB238989)

ab197013 staining ABCD1/ALD in the human cell line HeLa (human cervix adenocarcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

Isoytype control : Rabbit monoclonal IgG (Black)

Unlabelled control : Cell without incubation with primary antibody and secondary antibody (Blue)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab197013).

관련 conjugated 항체와 다양한 조성의 항체 (3)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15929

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Assay Kits

AB65341

Free Fatty Acid Assay Kit - Quantification

Biochemical assay - Free Fatty Acid Assay Kit - Quantification (AB65341)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB85550

Anti-PMP70 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMP70 antibody (AB85550)

  • 0
  • 0
Primary Antibodies

AB227455

Anti-Apolipoprotein A I antibody

Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody (AB227455)

  • 0
  • 0
Primary Antibodies

AB68190

Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Liver Carboxylesterase 1/CES1 antibody [EP1375Y] (AB68190)

  • 2
  • 1
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

제품 세부 정보

ab238989 is the carrier-free version of ab197013.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ABCD1 also known as the ABCD1 protein or ALD protein is encoded by the ABCD1 gene and belongs to the ATP-binding cassette (ABC) transporter family. It functions mechanically by transporting very long-chain fatty acids (VLCFAs) into peroxisomes where they can be metabolized. ABCD1 has a mass of approximately 80 kDa. Expression of ABCD1 is significant in tissues such as the liver adrenal glands and brain where it plays a role in lipid metabolism and cellular detoxification processes.
Biological function summary

ABCD1 contributes to the maintenance of normal fatty acid levels within cells. It is not known as part of a larger protein complex but it cooperates closely with other peroxisomal enzymes to facilitate the beta-oxidation of VLCFAs. This action ensures the breakdown of toxic compounds and has a significant role in maintaining cellular homeostasis. The proper function of ABCD1 is essential for preventing the accumulation of VLCFAs which can affect cellular function and viability.

Pathways

ABCD1 is integral to the metabolic pathway of peroxisomal beta-oxidation. This pathway is important for the degradation of excess VLCFAs which are otherwise difficult to break down in mitochondria. ABCD1 works in concert with related proteins such as ABCD2 and ABCD3 playing differentiated yet sometimes overlapping roles in lipid transport and metabolism. This cooperation is important for maintaining normal cellular metabolism and preventing lipid accumulation.

Defects in ABCD1 are directly associated with X-linked adrenoleukodystrophy (X-ALD) a neurodegenerative disorder characterized by the accumulation of VLCFAs in tissues. This condition leads to severe neurological impairment due to the toxic buildup of these lipids which disrupts normal brain function. Additionally the ABCD1 protein's dysfunction can indirectly affect the function of other proteins involved in lipid metabolism potentially exacerbating conditions related to peroxisomal disorders.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

ATP-dependent transporter of the ATP-binding cassette (ABC) family involved in the transport of very long chain fatty acid (VLCFA)-CoA from the cytosol to the peroxisome lumen (PubMed : 11248239, PubMed : 15682271, PubMed : 16946495, PubMed : 18757502, PubMed : 21145416, PubMed : 23671276, PubMed : 29397936, PubMed : 33500543). Coupled to the ATP-dependent transporter activity also has a fatty acyl-CoA thioesterase activity (ACOT) and hydrolyzes VLCFA-CoA into VLCFA prior their ATP-dependent transport into peroxisomes, the ACOT activity is essential during this transport process (PubMed : 29397936, PubMed : 33500543). Thus, plays a role in regulation of VLCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation, mitochondrial function and microsomal fatty acid elongation (PubMed : 21145416, PubMed : 23671276). Involved in several processes; namely, controls the active myelination phase by negatively regulating the microsomal fatty acid elongation activity and may also play a role in axon and myelin maintenance. Also controls the cellular response to oxidative stress by regulating mitochondrial functions such as mitochondrial oxidative phosphorylation and depolarization. And finally controls the inflammatory response by positively regulating peroxisomal beta-oxidation of VLCFAs (By similarity).
See full target information ABCD1
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature 590:344-350 PubMed33505024

2021

Integrated spatial genomics reveals global architecture of single nuclei.

Applications

Unspecified application

Species

Unspecified reactive species

Yodai Takei,Jina Yun,Shiwei Zheng,Noah Ollikainen,Nico Pierson,Jonathan White,Sheel Shah,Julian Thomassie,Shengbao Suo,Chee-Huat Linus Eng,Mitchell Guttman,Guo-Cheng Yuan,Long Cai
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com