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AB109395

Anti-Abi-1 (phospho Y435) antibody [EP3954]

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Rabbit Recombinant Monoclonal Abi-1 phospho Y435 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

SSH3BP1, ABI1, Abl interactor 1, Abelson interactor 1, Abl-binding protein 4, Eps8 SH3 domain-binding protein, Nap1-binding protein, Spectrin SH3 domain-binding protein 1, e3B1, Abi-1, AblBP4, Eps8-binding protein, Nap1BP

1 이미지
Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (AB109395)
  • WB

Lab

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (AB109395)

ab181602 was used a GAPDH loading control

All lanes:

Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395) at 1/10000 dilution

Lane 1:

Untreated Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Lane 2:

Raji treated with 1mM pervanadate for 30mins whole cell lysate at 15 µg

Lane 3:

Raji treated with 1mM pervanadate for 30mins whole cell lysate, then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 55 kDa

false

Exposure time: 7s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-Abi-1 (phospho Y435) antibody [EP3954] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP3954

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB65828

Anti-Abi-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (AB65828)

  • 0
  • 0
Primary Antibodies

AB72318

Anti-Abi-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (AB72318)

  • 0
  • 0
Primary Antibodies

AB233412

Anti-Abi-1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Abi-1 antibody (AB233412)

  • 0
  • 0

보완 제품

Primary Antibodies

AB82958

Anti-Bassoon/BSN antibody [SAP7F407]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bassoon/BSN antibody [SAP7F407] (AB82958)

  • 4
  • 12
Primary Antibodies

AB109305

Anti-UFM1 antibody [EPR4264(2)]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFM1 antibody [EPR4264(2)] (AB109305)

  • 5
  • 4
Primary Antibodies

AB184955

Anti-Homer1 antibody [EPR15309]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (AB184955)

  • 5
  • 2
Primary Antibodies

AB192757

Anti-PSD95 antibody [K28/43] - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody [K28/43] - Synaptic Marker (AB192757)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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제품 세부 정보

The production method for this product has been changed from hybridoma to recombniant on 16th April 2024

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Stable for 12 months at -20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Abi-1 protein also known as ABI family member 1 is a small adapter protein with a molecular mass of about 55 kDa. It is mainly expressed in brain and hematopoietic tissues. Abi-1 contains several domains allowing it to interact with a variety of signaling and cytoskeletal proteins. Its role as a linker connects various cellular components and facilitates communication between them important for the dynamic regulation of the actin cytoskeleton.
Biological function summary

Abi-1 influences actin polymerization and cell motility by associating with the WAVE complex. This complex plays an important function in mediating signal transduction from small GTPases to the actin cytoskeleton. Abi-1 helps to organize the cytoskeleton to enable cell movements and morphological changes. Moreover Abi-1 is involved in the regulation of signaling pathways linked with cell growth and differentiation. These functions position Abi-1 as an important factor in maintaining normal cellular function.

Pathways

Abi-1 operates inside the WAVE regulatory complex which affects the Rac1 signaling pathway. This pathway is essential for actin polymerization and cellular migration and involves proteins like N-WASP and Arp2/3 complex. Through its interaction with Rac1 and associated proteins Abi-1 greatly affects cytoskeletal reorganization demonstrating its importance in normal cellular movement and immune cell function.

Alterations in Abi-1 have associations with cancer particularly in leukemia. Abi-1 plays a role in oncogenic processes by interacting with proteins like BCR-ABL which is implicated in the pathogenesis of chronic myeloid leukemia. Additionally Abi-1 can contribute to neurological disorders due to its expression in brain tissues but the mechanisms remain less clearly understood. The disorder-associated signaling dynamics make Abi-1 a promising research focus for therapeutic interventions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons.
See full target information ABI1 pY435
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of clinical investigation 135: PubMed40794452

2025

Epigenetic targeting of PGBD5-dependent DNA damage in SMARCB1-deficient sarcomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yaniv Kazansky,Helen S Mueller,Daniel Cameron,Phillip Demarest,Nadia Zaffaroni,Noemi Arrighetti,Valentina Zuco,Prabhjot S Mundi,Yasumichi Kuwahara,Romel Somwar,Rui Qu,Andrea Califano,Elisa de Stanchina,Filemon S Dela Cruz,Andrew L Kung,Mrinal M Gounder,Alex Kentsis
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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