AB109368

Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]

20ul selling size
RabMAb
Recombinant
KO Validated
각 태그의 뜻

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(16 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal ACACA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 16 publications.

대체 명칭 보기

ACAC, ACC1, ACCA, ACACA, Acetyl-CoA carboxylase 1, Acetyl-Coenzyme A carboxylase alpha, ACC-alpha

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunohistochemical analysis of paraffin-embedded skeletal muscle tissue using unpurified ab109368 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunohistochemical analysis of paraffin-embedded brain tissue using unpurified ab109368 at 1/250 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunofluorescent staining of 293 cells using unpurified ab109368 at 1/100 dilution

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunocytochemistry/ Immunofluorescence analysis of 293 (Human embryonic kidney epithelial cell) cells labeling Acetyl Coenzyme A carboxylase with Purified ab109368 at 1 : 250 dilution (2.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Acetyl Coenzyme A carboxylase with purified ab109368 at 1 : 500 dilution (1.05 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer pH6.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

5% NFDM/TBST

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/5000 dilution

Lane 1:

293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 266 kDa

Observed band size: 266 kDa

false

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Lanes 1 - 4 : Merged signal (red and green). Green - ab109368 observed at 265 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab109368 was shown to specifically react with Acetyl Coenzyme A carboxylase in wild-type HAP1 cells as signal was lost in ACACA (Acetyl Coenzyme A Carboxylase) knockout cells. Wild-type and ACACA (Acetyl Coenzyme A Carboxylase) knockout samples were subjected to SDS-PAGE. ab109368 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

ACACA (Acetyl Coenzyme A Carboxylase) knockout HAP1 whole cell lysate at 20 µg

Lane 3:

Hela whole cell lysate at 20 µg

Lane 4:

A431 whole cell lysate at 20 µg

Predicted band size: 266 kDa

false

Unknown

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (ab109368) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 266 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4971

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/1000- 1/10000.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/1000- 1/10000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/1000- 1/10000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Acetyl Coenzyme A Carboxylase (ACC) also known as acetyl-CoA carboxylase or ACAC is an enzyme that plays an important role in fatty acid metabolism. Mechanically it catalyzes the biotin-dependent carboxylation of acetyl coenzyme A (acetyl-CoA) to produce malonyl-CoA which is an important precursor in the biosynthesis of fatty acids. The molecular weight of ACC is approximately 265 kDa. Humans express this enzyme in multiple tissues such as the liver adipose tissue and mammary glands.

Biological function summary

Acetyl Coenzyme A Carboxylase contributes to fatty acid synthesis and regulation of metabolism. ACC exists in two main isoforms ACC1 which is found mainly in lipogenic tissues and ACC2 which is associated with oxidative tissues. These isoforms form part of larger complexes within the cell interacting with other enzymes and proteins to regulate metabolic processes. ACC also affects the synthesis of long-chain fatty acids by regulating the amount of malonyl-CoA available as a building block.

Pathways

Acetyl Coenzyme A Carboxylase plays a role in the synthesis of fatty acids and their cellular metabolism. This enzyme is a component of the lipogenesis pathway where it transforms acetyl-CoA to malonyl-CoA a step critical for fatty acid elongation. ACC interacts with proteins such as fatty acid synthase to carry out its function within these metabolic pathways. Additionally malonyl-CoA produced by ACC serves as a regulator of carnitine palmitoyltransferase 1 integrating with the fatty acid oxidation pathway.

Alterations in the function of acetyl Coenzyme A Carboxylase link to conditions like obesity and type 2 diabetes. Overexpression of ACC can result in increased fat storage contributing to obesity while its inhibition has been considered a strategy to counter insulin resistance in diabetes. In cancer dysregulation of ACC especially ACC1 can lead to altered lipid synthesis promoting tumor growth. ACC1 interacts with other proteins such as AMP-activated protein kinase (AMPK) which senses energy status and is involved in the regulation of ACC activity thereby influencing these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed : 20457939, PubMed : 20952656, PubMed : 29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed : 20457939, PubMed : 20952656, PubMed : 29899443).

See full target information ACACA

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제품이 사용된 논문 (16)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer cell international 25:25 PubMed39871246

2025

DLAT is involved in ovarian cancer progression by modulating lipid metabolism through the JAK2/STAT5A/SREBP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,Shen Luo,Yue Yin,Yang Liu,Xiaomei Sun,Ling Qiu,Xin Wu

Open life sciences 19:20221007 PubMed39711976

2024

ZAG promotes colorectal cancer cell proliferation and epithelial-mesenchymal transition by promoting lipid synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Maotao Xu,Xingzheng Jin,Zhouli Shen

Scientific reports 14:28258 PubMed39550424

2024

A novel role of peroxiredoxin 2 in diabetic kidney disease progression by activating the classically activated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xia Li,Hehua Long,Rui Peng,Xue Zou,Siyang Zuo,Yuan Yang,Min Chen,Huixiong Yuan,Zeying Liu,Teng Wang,Qingqing Zhao,Bing Guo,Lirong Liu

PeerJ 12:e16740 PubMed38274331

2024

Regulation of lipid metabolism by APOE4 in intrahepatic cholangiocarcinoma the enhancement of ABCA1 membrane expression.

Applications

Unspecified application

Species

Unspecified reactive species

Liqiang Qian,Gang Wang,Bin Li,Haoyuan Su,Lei Qin

Journal of animal science 101: PubMed37367933

2023

Lnc-TRTMFS promotes milk fat synthesis via the miR-132x/RAI14/mTOR pathway in BMECs.

Applications

Unspecified application

Species

Unspecified reactive species

Hongru Jia,Zhangqing Wu,Jianbing Tan,Silin Wu,Chaoqun Yang,Sayed Haidar Abbas Raza,Meng Wang,Guibing Song,Yujie Shi,Linsen Zan,Wucai Yang

Cancer cell international 23:83 PubMed37120513

2023

Imaging the metabolic reprograming of fatty acid synthesis pathway enables new diagnostic and therapeutic opportunity for breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Fukai Wang,Shuangshuang Ma,Panpan Chen,Yuhao Han,Zhaoyun Liu,Xinzhao Wang,Chenglong Sun,Zhiyong Yu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e2204711 PubMed36307901

2022

Upregulation of Superenhancer-Driven LncRNA FASRL by USF1 Promotes De Novo Fatty Acid Biosynthesis to Exacerbate Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Yun Peng,Dian-Kui Cai,Ren-Li Zeng,Chao-Yang Zhang,Guan-Cheng Li,Si-Fan Chen,Xiao-Qing Yuan,Li Peng

Cell death discovery 8:401 PubMed36180463

2022

ACE2 deficiency exacerbates obesity-related glomerulopathy through its role in regulating lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Yin-Yin Che,Han Hong,Yu-Ting Lei,Jia Zou,Yi-Ya Yang,Li-Yu He

Medical oncology (Northwood, London, England) 39:202 PubMed36175596

2022

CD36 accelerates the progression of hepatocellular carcinoma by promoting FAs absorption.

Applications

Unspecified application

Species

Unspecified reactive species

Lide Tao,Xiangmin Ding,Lele Yan,Guangcai Xu,Peijian Zhang,Anlai Ji,Lihong Zhang

Frontiers in bioengineering and biotechnology 10:858558 PubMed35769097

2022

LncRNA MALAT1 Promotes PPARα/CD36-Mediated Hepatic Lipogenesis in Nonalcoholic Fatty Liver Disease by Modulating miR-206/ARNT Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xiang,Yuan-Yuan Deng,Hui-Xia Liu,Ying Pu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

Western blot - Anti-Acetyl Coenzyme A Carboxylase antibody [EPR4971] (AB109368)

관련 conjugated 항체와 다양한 조성의 항체 (6)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (16)

Product promise