AB68191

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]

Name: Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]
Brand: Abcam Limited
SKU: AB68191
Rating: 5 (1 reviews)

20ul selling size
RabMAb
Recombinant
KO Validated
각 태그의 뜻

(1 리뷰)

(38 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal ACACA phospho S79 antibody. Suitable for Dot, WB and reacts with Mouse, Rat, Human samples. Cited in 38 publications.

5 이미지

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Lanes 1 - 3 : Merged signal (red and green). Green - ab68191 observed at 266 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab68191 was shown to specifically react with in wild-type HAP1 cells as signal was lost in ACACA knockout cells. Wild-type and ACACA knockout samples were subjected to SDS-PAGE. ab68191 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/5000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

ACACA knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HEK293 whole cell lysate at 20 µg

Predicted band size: 266 kDa

Observed band size: 266 kDa

false

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/10000 dilution

Lane 1:

Untreated A431 whole cell lysate at 10 µg

Lane 2:

A431 whole cell lysate - treated with lambda phosphatase at 10 µg

Secondary

All lanes:

Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/2000 dilution

Predicted band size: 266 kDa

Observed band size: 265 kDa

false

Exposure time: 30s

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate, Then the membrane was incubated with phosphatase at 10 µg

Lane 2:

Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 266 kDa

Observed band size: 265 kDa

false

Exposure time: 5s

Lab

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate, Then the membrane was incubated with phosphatase at 10 µg

Lane 2:

Untreated RAW264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 266 kDa

Observed band size: 265 kDa

false

Exposure time: 8s

Unknown

Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Dot blot analysis of Acetyl Coenzyme A Carboxylase (pS79) phospho peptide (lane 1) and Acetyl Coenzyme A Carboxylase non-phospho peptide (lane 2) labelling Acetyl Coenzyme A Carboxylase (phospho S79) with ab68191 at a dilution of 1/1000. A peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/2500).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1885Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Acetyl Coenzyme A Carboxylase (ACC) also known as acetyl-CoA carboxylase or ACAC is an enzyme that plays an important role in fatty acid metabolism. Mechanically it catalyzes the biotin-dependent carboxylation of acetyl coenzyme A (acetyl-CoA) to produce malonyl-CoA which is an important precursor in the biosynthesis of fatty acids. The molecular weight of ACC is approximately 265 kDa. Humans express this enzyme in multiple tissues such as the liver adipose tissue and mammary glands.

Biological function summary

Acetyl Coenzyme A Carboxylase contributes to fatty acid synthesis and regulation of metabolism. ACC exists in two main isoforms ACC1 which is found mainly in lipogenic tissues and ACC2 which is associated with oxidative tissues. These isoforms form part of larger complexes within the cell interacting with other enzymes and proteins to regulate metabolic processes. ACC also affects the synthesis of long-chain fatty acids by regulating the amount of malonyl-CoA available as a building block.

Pathways

Acetyl Coenzyme A Carboxylase plays a role in the synthesis of fatty acids and their cellular metabolism. This enzyme is a component of the lipogenesis pathway where it transforms acetyl-CoA to malonyl-CoA a step critical for fatty acid elongation. ACC interacts with proteins such as fatty acid synthase to carry out its function within these metabolic pathways. Additionally malonyl-CoA produced by ACC serves as a regulator of carnitine palmitoyltransferase 1 integrating with the fatty acid oxidation pathway.

Alterations in the function of acetyl Coenzyme A Carboxylase link to conditions like obesity and type 2 diabetes. Overexpression of ACC can result in increased fat storage contributing to obesity while its inhibition has been considered a strategy to counter insulin resistance in diabetes. In cancer dysregulation of ACC especially ACC1 can lead to altered lipid synthesis promoting tumor growth. ACC1 interacts with other proteins such as AMP-activated protein kinase (AMPK) which senses energy status and is involved in the regulation of ACC activity thereby influencing these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis (PubMed : 20457939, PubMed : 20952656, PubMed : 29899443). This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA (PubMed : 20457939, PubMed : 20952656, PubMed : 29899443).

See full target information ACACA pS79

대체 명칭 보기

ACAC, ACC1, ACCA, ACACA, Acetyl-CoA carboxylase 1, Acetyl-Coenzyme A carboxylase alpha, ACC-alpha

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제품이 사용된 논문 (38)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Iranian journal of basic medical sciences 28:31-37 PubMed39877628

2025

Effects of different pre-conditioning exercise on leptin synthesis and its downstream signalling pathway in T2DM rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sen Lin,Yuzhi Hu,Shuqiao Ding,Yazhe Hu

Journal of oleo science 72:929-938 PubMed37704444

2023

Selenium-enriched Polysaccharides from Pyracantha fortuneana (SePFP) Ameliorated Hepatic Lipid Accumulation through Enhancing Mitochondrial Fatty Acid β-Oxidation in Aging Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Hu,Rui Wang,Shuwen Wang,Chengfu Yuan,Qi Yuan,Yifan Zhang,Jianxia Ren,Yumin He,Xiaoshan Wu,Wenjun Dai,Wenyong Wang,Shijun Xie

Molecular medicine (Cambridge, Mass.) 29:33 PubMed36918760

2023

Caveolin-1 is involved in fatty infiltration and bone-tendon healing of rotator cuff tear.

Applications

Unspecified application

Species

Unspecified reactive species

Shanhong Fang,Mengqiang You,Jie Wei,Peng Chen

Molecular biology reports 50:2591-2601 PubMed36626064

2023

Intramuscular mitochondrial and lipid metabolic changes of rats after regular high-intensity interval training (HIIT) of different training periods.

Applications

Unspecified application

Species

Unspecified reactive species

Ruonan Shangguan,Zhiqiang Hu,Yuzhen Luo,Min Chen,Xiangdeng Lai,Jingquan Sun,Siyu Chen

Phytotherapy research : PTR 36:2495-2510 PubMed35445769

2022

Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.

Applications

Unspecified application

Species

Unspecified reactive species

Long Cheng,Lu Shi,Changhao He,Chen Wang,Yinglan Lv,Huimin Li,Yongcheng An,Hongyu Dai,Yuhui Duan,Huilin Zhang,Yan Huang,Wanxin Fu,Yanyan Meng,Baosheng Zhao

Bioengineered 13:8349-8359 PubMed35311465

2022

Nuclear receptor 4A1 (NR4A1) silencing protects hepatocyte against hypoxia-reperfusion injury by activating liver kinase B1 (LKB1)/AMP-activated protein kinase (AMPK) signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zheng,Yingying Tao,Xiaobo Zhan,Qi Wu

Nature communications 13:1172 PubMed35246531

2022

Oxygen-sensitive methylation of ULK1 is required for hypoxia-induced autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyi Li,Tao Zhang,Tao Ren,Xiaoyu Liao,Yilong Hao,Je Sun Lim,Jong-Ho Lee,Mi Li,Jichun Shao,Rui Liu

Frontiers in cardiovascular medicine 9:803510 PubMed35282369

2022

Exercise-Generated β-Aminoisobutyric Acid (BAIBA) Reduces Cardiomyocyte Metabolic Stress and Apoptosis Caused by Mitochondrial Dysfunction Through the miR-208b/AMPK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Yu,Wewei Chen,Ming Yu,Jinsha Liu,Huan Sun,Ping Yang

Frontiers in physiology 12:709135 PubMed34588991

2021

The Role of cAMP-PKA Pathway in Lactate-Induced Intramuscular Triglyceride Accumulation and Mitochondria Content Increase in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Siyu Chen,Lei Zhou,Jingquan Sun,Yaqian Qu,Min Chen

Journal of cellular and molecular medicine 25:8863-8876 PubMed34402182

2021

Astragaloside IV ameliorates fat metabolism in the liver of ageing mice through targeting mitochondrial activity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixiao Luo,Yaqi Wang,Mengzhen Xue,Fangqi Xia,Leiqi Zhu,Yuanyang Li,Dengke Jia,Silong Chen,Guangfu Xu,Yan Lei

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y]

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Western blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

Dot Blot - Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (AB68191)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (38)

Product promise