AB238913

Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free

RabMAb
Recombinant
KO Validated
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal PPAC antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

대체 명칭 보기

Low molecular weight phosphotyrosine protein phosphatase, LMW-PTP, LMW-PTPase, Adipocyte acid phosphatase, Low molecular weight cytosolic acid phosphatase, Red cell acid phosphatase 1, ACP1

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Acid Phosphatase with ab235449 at 1/100 dilution, followed by ab150077 Alexa-Fluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HeLa cell line (PMID 26159288) is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used as the counterstain (Red). The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

Flow Cytometry (Intracellular) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cell line labeling Acid Phosphatase with ab235449 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control, and an unlabeled control (Cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Acid Phosphatase with ab235449 at 1/100 dilution, followed by ab150077 Alexa-Fluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Confocal image showing cytoplasmic and nuclear staining in HepG2 cell line (PMID 26159288) is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used as the counterstain (Red). The nuclear counterstain is DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunohistochemical analysis of paraffin-embedded human prostatic hyperplasia tissue labeling Acid Phosphatase with ab235449 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human prostatic hyperplasia (PMID : 26159288) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235449).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling Acid Phosphatase with ab235449 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human colon cancer (PMID : 25811796) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP)ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab235449).

Lab

Western blot - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

This data was developed using the same antibody clone in a different buffer formulation (ab235449).

Lanes 1 - 4 : Merged signal (red and green). Green - ab235449 observed at 18 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab235449 was shown to react with Acid phosphatase in wild-type HEK-293 cells in western blot with loss of signal observed in ACP1 knockout sample. Wild-type and ACP1 knockout HEK-293 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab235449 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Acid phosphatase antibody [EPR21791] (<a href='/ko/products/primary-antibodies/acid-phosphatase-antibody-epr21791-ab235449'>ab235449</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

ACP1 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ACP1 (Acid phosphatase) knockout HEK-293 cell line (<a href='/ko/products/cell-lines/human-acp1-acid-phosphatase-knockout-hek-293-cell-line-ab261859'>ab261859</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21791

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab238913 is the carrier-free version of ab235449.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Acid phosphatase also known as tartrate-resistant acid phosphatase (TRAP) or prostatic acid phosphatase (PAP) is an enzyme that catalyzes the hydrolysis of phosphate esters in an acidic environment. It has a molecular mass of approximately 50 kDa and is expressed in various human tissues including the prostate bone liver and spleen. Within the prostate it breaks down and releases phosphate from different compounds and it plays a role in bone resorption by osteoclasts.

Biological function summary

This enzyme contributes to multiple cellular processes including the regulation of bone metabolism and prostate health. Acid phosphatase when acting as TRAP is often found within osteoclast lysosomes where it degrades bone matrix components. It does not form part of a complex but functions independently to modulate these processes. Prostatic acid phosphatase in seminal fluid has additional roles particularly in prostate tissue affecting cellular proliferation and motility.

Pathways

Acid phosphatase participates in both bone resorption and lysosomal degradation pathways. In bone metabolism it works alongside proteins such as cathepsin K assisting in the breakdown of matrix constituents during bone remodeling. In lysosomal pathways it complements activities of enzymes like lysosomal acid lipase helping in recycling cellular materials. These interactions underline its involvement in maintaining cellular homeostasis through these critical biological pathways.

Acid phosphatase relates significantly to prostate cancer and bone disorders like osteoporosis. Elevated levels of prostatic acid phosphatase in serum serve as a marker for prostate cancer linking it to androgen receptor pathways critical in disease progression. In bone disorders TRAP's role in osteoclast function connects it to conditions such as osteoporosis where its interaction with integrin receptor influences bone density regulation. Understanding these connections can aid in developing diagnostic and therapeutic strategies for these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates with differences in substrate specificity between isoform 1 and isoform 2.. Isoform 3. Does not possess phosphatase activity.

See full target information ACP1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of biological sciences 20:1045-1063 PubMed38322121

2024

The homeoprotein HOXB2 limits triple-negative breast carcinogenesis via extracellular matrix remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hoon Oh,Clara Yuri Kim,Da Som Jeong,Yu Cheon Kim,Myoung Hee Kim,Je-Yoel Cho

Annals of translational medicine 9:1474 PubMed34734026

2021

Induction of osteoclast formation by LOX mutant (LOXG473A) through regulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Chenglin Luo,Wenjin Xiao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Flow Cytometry (Intracellular) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunocytochemistry/ Immunofluorescence - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

Western blot - Anti-Acid phosphatase antibody [EPR21791] - BSA and Azide free (AB238913)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise