AB216070

Anti-Actin antibody [EPR19353-16] - Loading Control

20ul selling size
RabMAb
Recombinant
Lab Essentials
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal alpha skeletal muscle Actin antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

대체 명칭 보기

ACTA, ACTA1, Alpha-actin-1

5 이미지

Supplier Data

Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Actin was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab216070 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216070 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution

Lane 1 : NIH/3T3 whole cell lysate, 10 μg (Input).

Lane 2 : ab216070 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab216070 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

Note : Customer optimization may be required.

All lanes:

Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - Loading Control (ab216070)

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (ab216070) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (ab216070) at 1/1000 dilution

Lane 1:

Human skeletal muscle lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/4000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Blocking/Dilution buffer : 5% NFDM/TBST.

Human Gamma actin (ACTG1) contains aa2-182 with His-tag. Human Beta actin (ACTB) contains aa1-100 with GST and His tag. Human Gamma-2 actin (ACTG2) contains aa1-200 with His-tag. Human Alpha-cardiac actin (ACTC) contains aa1-200 with His-tag. Human Alpha-actin-2 (ACTA2) contains aa3-253 with His-tag. Human Alpha-actin-1 (ACTA1) contains aa3-200 with His-tag.

All lanes:

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (ab216070) at 1/1000 dilution

Lane 1:

Human Gamma actin (ACTG1) recombinant protein at 0.01 µg

Lane 2:

Human Beta actin (ACTB) recombinant protein at 0.02 µg

Lane 3:

Human Gamma-2 actin (ACTG2) recombinant protein at 0.02 µg

Lane 4:

Human Alpha-cardiac actin (ACTC) recombinant protein at 0.02 µg

Lane 5:

Human Alpha-actin-2 (ACTA2) recombinant protein at 0.01 µg

Lane 6:

Human Alpha-actin-1 (ACTA1) recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (ab216070) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Lane 4:

Mouse brain lysate at 10 µg

Lane 5:

Mouse spleen lysate at 10 µg

Lane 6:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19353-16

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This is a pan-actin antibody. It will detect all 6 actin isoforms corresponding to SwissProt IDs P63261, P60709, P68032, P63267, P62736 and P68133.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Actin also known as globular (G-actin) or filamentous (F-actin) actin plays a central role in cell structure and movement. This protein has a molecular weight of approximately 42 kDa and resides abundantly in eukaryotic cells especially in muscle and cytoskeletal structures. It comes in several isoforms with varying expression profiles across tissues. Actin can undergo polymerization from its G-actin monomers into F-actin filaments a process that is reversible through depolymerization allowing for dynamic cellular functions.

Biological function summary

Actin contributes to various cellular processes by forming the cytoskeleton which provides mechanical support and determines cell shape. Actin also facilitates cell motility division and intracellular transport through rapid polymerization and depolymerization cycles. Within cells actin associates with other proteins to form complexes such as the Arp2/3 complex which assists actin in the branching of filaments critical for pushing forward the cell's leading edge during movement. Techniques like actin immunofluorescence help visualize actin filaments within cells revealing its extensive network.

Pathways

Actin plays an integral role in pathways like cell movement and signal transduction. The Rho family of GTPases regulates actin cytoskeleton rearrangements influencing cell shape and migration. Actin function interactions involve proteins like myosin forming actomyosin complexes essential for muscle contraction and other cell motility activities. Actin polymerization and depolymerization cycles are key to the dynamic regulation within these pathways ensuring adequate cellular responses to environmental signals.

Mutations or misregulation of actin and its associated pathways link to conditions such as cardiomyopathies and cancer metastasis. In familial cardiomyopathy actin mutations disrupt normal cardiac muscle contraction. In cancer altered actin polymerization and depolymerization enable invasive cell migration facilitating metastasis. Proteins like myosin and tropomyosin associate with actin in these pathologies illustrating the impact of actin dynamics on disease progression and highlighting actin as a therapeutic target. Anti-actin antibodies can be used in research and diagnostics to better understand these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

BMC cardiovascular disorders 25:141 PubMed40021969

2025

Identification of the CeRNA axis of circ_0000006/miR-483-5p/KDM2B in the progression of aortic aneurysm to aorta dissection.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Liu,Xiong Tan,Liang Wang,Weitao Jin,Yangchen He,Yu Yan,Kai Hu,Hao Wang,Chaohu Xiang,Ming Hou,Yinglong Lai

PloS one 19:e0304668 PubMed38820386

2024

Circ_0000006 and circ_0000160 regulate hsa-let-7e-5p/UBQLN4 axis in aortic dissection progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Liu,Liang Wang,Dongyun Lei,Xiong Tan,Weitao Jin,Ming Hou,Kai Hu,Yu Yan,Hao Wang,Chaohu Xiang,Yinglong Lai

Molecular and cellular biology 42:e0039721 PubMed35862727

2022

MicroRNA-27a-5p Inhibits Proliferation, Migration, and Invasion and Promotes Apoptosis of Wilms' Tumor Cell by Targeting PBOV1.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng-Tuan Guo,Qiang Yu,Chunlin Miao,Wenan Ge,Peng Li

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Actin antibody [EPR19353-16] - Loading Control

Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

Western blot - Anti-Actin antibody [EPR19353-16] - Loading Control (AB216070)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise