Anti-Actin antibody [IGX3831R-3] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831R-3] - BSA and Azide free (AB325980)

This data was developed using ab218787, the same antibody clone in a different buffer formulation.

ab218787 staining Actin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min) permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab218787 at a 5µg/ml concentration then detected with a goat anti-human (Alexa Fluor^® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Actin antibody [IGX3831R-3] - BSA and Azide free (AB325980)

This data was developed using ab218787, the same antibody clone in a different buffer formulation.

ab218787 staining Actin in NIH3T3 cells. The cells were fixed with 4% formaldehyde (10 min) permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab218787 at a 5µg/ml concentration then detected with a goat anti-human (Alexa Fluor^® 488) secondary antibody at a 1/2000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• WB

Lab

Western blot - Anti-Actin antibody [IGX3831R-3] - BSA and Azide free (AB325980)

All lanes:

Western blot - Anti-Actin antibody [IGX3831R-3] - Loading Control (<a href='/ko/products/primary-antibodies/actin-antibody-igx3831r-3-loading-control-ab218787'>ab218787</a>) at 1 µg/mL

Lane 1:

Human colon tissue lysate at 10 µg

Lane 2:

Mouse colon tissue lysate at 10 µg

Lane 3:

HeLa whole cell lysate at 10 µg

Lane 4:

Jurkat whole cell lysate at 10 µg

Lane 5:

NIH3T3 whole cell lysate at 10 µg

Lane 6:

PANC1 whole cell lysate at 10 µg

Lane 7:

C2C12 whole cell lysate at 10 µg

Lane 8:

Human skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution

Predicted band size: 42 kDa

true

Exposure time: 2min